Malaysian Applied Biology Journal

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05_UmikalsomY

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Malays. Appl. Biol. (December 2003) 32(2): 35-39

GENOTOXIC EFFECT OF FLAVONOID OF CARTHAMUS TINCTORIUS IN MITOTIC CELLS OF ALLIUM CEPA

UMIKALSOM, Y.1, KHAIRUDDIN, H.I.2, SINNIYAH, S.2, ABDULLAH, F.1, HASSAN, U.S.1, SUKARI, M.A.3 and SIM, L.P.1

Department of Biology, Universiti Putra Malaysia, 43400, Serdang, Malaysia

Institute of Bioscience, University of Malaya, 50603, Kuala Lumpur, Malaysia

Department of Chemistry, Universiti Putra Malaysia, 43400, Serdang, Malaysia

ABSTRACT

The cytological effects (induction of mitotic chromosome aberrations in root tip cells) of flavonoid from the flower oi C. tinctorius on Allium cepa root meristems were studied. The flavonoid quercetin-3-glucoside, quercetin-3-rutinoside, kaempferol-3-glucoside and kaempferol-3-rutinoside were found to be mitodepressive and induced statistically significant chromosome aberrations in root cells. Abnormalities scored were stickiness, c-mitosis, clumping, micronuclei, lagging and bridges.

ABSTRAK

Kajian telah dijalankan terhadap kesan sitologi (induksi aberasi terhadap kromosom mitotic di dalam sel-sel hujung akar) oleh flavonoid dari bunga Carthamus tinctorius ke atas meristem akar Allium cepa. Flavonoid kuersetin 3-glukosida, kuersetin 3-rutinosida, kaemferol 3-glukosida dan kaemferol 3-rutinosida telah didapati sebagai perencat mitotic dar menginduksikan aberasi kromosom di dalam sel-sel akar dengan aras keertian statistic yang signifikan. Ketidaknormalan kromosom yang ditunjukkan adalah seperti kelekitan, pengelompokan, mikronukleus, bebatan dan jejambat.

Key words: Carthamus tinctorius, flavonoid glycosides, cytotoxicity.

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