Malaysian Applied Biology Journal

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Malays. Appl. Biol. (December 2002) 31(2): 37-45


YAP, C. K.,* ISMAIL, A. and TAN, S. G.

Department of Biology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor, Malaysia.


The relationship between Condition Index (CI) and accumulated concentrations of Cd and Pb were determined in field samples of the green-lipped mussel Perna viridis and under experimental conditions. The field samples included those from uncontaminated and contaminated sites. In the field samples, significant (P< 0.05) negative relationships between CI and metal concentrations were found. However, the results could also be due to variation in nutritional state and reproductive status of the mussels. To clarify these uncertainties, an experimental study was conducted and the results agreed with that found in the field samples. Therefore, the results indicated that if the heavy metal levels of the field collected mussels were significantly (P< 0.001) different, the influence of the nutritional and reproductive states would be masked by the ecotoxicological effects of heavy metal contamination, provided that mussels with similar size range were selected for the CI determination. The CI can be used to show that P. viridis is sensitive to Cd and Pb.


Hubungkait di antara 'Indeks Kondisi' (CI) dan pengumpulan penimbunan kepekatan bagi kadmium dan plumbum telah ditentukan di dalam kupang Perna viridis dari lapangan dan yang di bawah keadaan eksperimen. Sampel lapangan adalah daripada kawasan tidak tercemar dan yang tercemar. Di dalam sampel lapangan, hubungan negatif yang signifikan (P< 0.05) di antara CI dan kepekatan logam telah diperolehi. Akan tetapi, keputusan tersebut boleh juga disebabkan oleh perubahan keadaan nutrisi dan status pembiakan bagi kupang tersebut. Untuk menjelaskan lagi ketidakpastian tersebut, satu eksperimen telah dijalankan dan keputusan ini mempersetujui apa yang diperolehi di dalam sampel lapangan. Oleh demikian, keputusan ini menunjukkan sekiranya logam berat dalam kupang dari lokasi berlainan mempunyai tahap logam berat yang signifikan (P< 0.001), pengaruh disebabkan oleh nutrisi dan status pembiakan akan menjadi tidak penting disebabkan oleh pengaruh ekotoksikologi pencemaran logam berat yang lebih kuat, dengan syarat julat saiz kupang yang dipilih haruslah sama untuk penentuan CI. CI boleh digunakan untuk menunjukkan P. viridis adalah organism yang sensitif kepada kadmium dan plumbum.

Key words: Heavy Metals, Condition Index, Perna viridis, Peninsular Malaysia


Akberali, H. B. and Trueman, E. R. 1985. Effects of environmental stress on marine bivalve molluscs. Advances in Marine Biology, 22: 102-198.

Bayne, B. L. 1973. Physiological changes in Mytilus edulis L. induced by temperature and nutritive stress. Journal of the Marine Biological Association of the United Kingdom, 53: 39-58.

Broman, D., Lindqvist, L. and Lundbergh, I. 1991. Cadmium and zinc in Mytilus edulis (L.) from the Bothnian and the Northern Baltic Proper. Environmental Pollution, 74: 227-244.

Chapman, P. M. 1995. Extrapolating laboratory toxicity tests to the field. Environmental Toxicology and Chemistry, 14: 927-930.

Cheung, S. G. 1993. The physiological ecology of '•Perna viridis (Bivalvia: Mytilidae) from contrasting environments in Hong Kong. In: Proceedings of the first International Conference on the Marine Biology of Hong Kong and the South China Sea, 1990, Hong Kong. B. Morton (ed.). Hong Kong University Press, pp. 559-579.

Cheung, V. V., Wedderburn, R. J. and Depledge, M. H. 1998. Molluscan lysosomal responses as a diagnostic tool for the detection of a pollution gradient in Tolo Harbour, Hong Kong. Marine Environmental Research, 46: 1-5.

Chusheng, W., Xiulan, Z. and Rongzhong, C. 1993. The effects of pollutants on the filtration rate of Perna viridis (Bivalvia: Mytilidae). In: Proceedings of the first International Conference on the Marine Biology of Hong Kong and the South China Sea, 1990, Hong Kong. B. Morton (ed.). Hong Kong University Press, pp. 253-260.

Cotter, A. J. R., Phillips, D. J. H. and Ahsanullah, M. 1982. The significance of temperature, salinity and zinc as lethal factors for the mussel Mytilus edulis in a polluted estuary. Marine Biology, 68: 135-141.

George, S. G. and Pirie, B. J. S. 1980. Metabolism of zinc in the mussel Mytilus edulis (L): a combined ultrastructural and biochemical study. Journal of the Marine Biological Association of the United Kingdom, 60: 575-590.

Goldberg, E. D. 1975. The mussel watch - a first step in global marine monitoring. Marine Pollution Bulletin, 6: 111.

Granby, K. and Spliid, N. H. 1995. Hydrocarbon and organochlorines in common mussels from the Kattegat and the Belts and their relation to condition indices. Marine Pollution Bulletin, 30: 74-82.

Hummel, H., Modderman, R., Amiard-Triquet, C., Rainglet, F., van Duijn, Y., Herssevoort, M., de Jong, J., Bogaards, R., Bachelet, G., Desprez, M., Marchand, J., Sylvand, B., Amiard, J-C., Rybarczyk, H. and de Wolf, L. 1997. A. comparative study on the relation between copper and condition in marine bivalves and the relation with copper in the sediment. Aquatic Toxicity, 38: 165-181.

Ismail, A., Badri, M. A. and Ramlan, M. N. 1991. Heavy metals contamination in fiddler crabs (Uca annulipes) and hermit crabs (Clibanarius sp.) in a coastal area of northern Peninsular Malaysia. Environmental Technology, 12: 923-926.

Ismail, A., Yap, C. K., Zakaria, M. P., Tanabe, S., Takada, H. and Ismail, A. R. 2000. Green-lipped mussel Perna viridis (L.) as a biomonitoring agent for heavy metals in the west coast of Peninsular Malaysia. In: Proceedings of the first International Conference on the Straits of Malacca, 1999, Malacca. M. Shariff, F. M. Yusoff, N. Gopinath, H. M. Ibrahim and A. Nik Mustapha (eds.). Malacca Straits Research and Development Centre (MASDEC), University Putra Malaysia, Serdang, Malaysia, pp. 553-559.

Lares, M. L. and K. J. Orians. 1997. Natural Cd and Pb variations in Mytilus californianus during the upwelling season. Science of the Total Environment, 197: 177-195.

Leavitt, D. F., Lancaster, B. A., Lancaster, A. S. and Capuzzo, J. M. 1990. Changes in the biochemical composition of a subtropical bivalve, Area zebra, in response to contaminant gradients in Bermuda. Journal of Experimental Marine Biology and Ecology, 138: 85-98.

Lobel, P. B., Bajdik, C. D., Jackson, S. E. and Longerich, H. P. 1991. Improved protocol for collecting mussel watch specimens taking into account sex, size, condition, shell, shape and chronological age. Archives of Environmental Contamination and Toxicology, 21: 409-414.

Lucas, A. and Beninger, P. G. 1985. The use of physiological condition indices in marine bivalve aquaculture. Aquaculture, 44: 187-200.

Marcus, J. M., Scott, G. I. and Heizer, D. D. 1989. The use of oyster shell thickness and CI measurements as physiological indicators of heavy metal pollution around three coastal marinas. Journal of Shellfish Research, 8: 87-94.

Martin M., Ichikawa, G., Goetzel, J., Reyes, M. de los. and Stephenson, M. D. 1984. Relationships between physiological stress and trace toxic substances in the bay mussel Mytilus edulis from San Francisco Bay, California. Marine Environmental Research, 11: 91-110.

Mo, C. and Neilson, B. 1994. Standardization of oyster soft dry weight measurements. Water Research, 28: 243-246.

Nicholson, S. 1999. Cytological and physiological biomarker responses from green mussels, Perna viridis (L.) transplanted to contaminated sites in Hong Kong coastal waters. Marine Pollution Bulletin, 39: 261-268.

Page, D. S., Gilfillan, E. S., Hanson, S. A., Hotham, J. R. and Foster, J. C. 1984. Correlation of body burden of heavy metals with physiological stress indicators in Mytilus edulis and Mya arenaria exposed to heavy metals in the field. Marine Environmental Research, 14: 505.

Peddicord, R. K. 1977. Salinity and substratum effects on condition index of the bivalve Rangia cuneata. Marine Biology, 39: 351-360.

Phillips, D. J. H. and Rainbow, P. S. 1993. Biomonitoring of Trace Aquatic Contaminants. Elsevier Science Publishers LTD, London. 371pp.

Phillips, D. J. H. and Segar, D. A. 1986. Use of bioindicator organism to monitoring contaminants: programme design imperatives. Marine Pollution Bulletin, 17: 10-17.

Phillips, D. J. H. 1980. Quantitative Aquatic Biological Indicators: Their Use to Monitor Trace Metal and Organochlorine Pollution. Applied Science Publishers, London. 488pp.

Phillips, D. J. H. 1991. Selected trace elements and the use of biomonitors in subtropical and tropical marine ecosystems. Reviews of Environmental Contamination and Toxicology, 120: 105- 129.

Regoli, F. and Principato, G. 1995. Glutathione, glutathione-dependent and anti-oxidant enzymes in Mussels, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical markers. Aquatic Toxicity, 31: 143-164.

Roberts, D. F., Elliot, M. and Read, P. A. 1986. Cadmium contamination, accumulation and some effects of this metals in mussels from a polluted marine environment. Marine Environmental Research, 18: 165-183.

Roesijadi, G., Young, Y. S., Drum, A. S. and Gurtisen, J. M. 1984. Behaviour of trace metals in Mytilus edulis during a reciprocal transplant field experiment. Marine Ecology Progress Series, 18: 155-170.

Sze, P. W. C. and Lee, S. Y. 1995. The potential role of mucus in the depuration of copper from the mussels Perna viridis (L.) and Septifer virgatus (Wiegmann). Marine Pollution Bulletin, 31: 390-395.

Sze, P. W. C. and Lee, S. Y. 2000. Effects of chronic copper exposure on the green mussel Perna viridis. Marine Biology, 137: 379-392.

Viarengo, A., Palmero, S., Zanicchi, G., Capelli, R. Vaissiere, R. and Orunesu, M. 1985. Role of metallothioneins in Cu and Cd accumulation and elimination in the gill and digestive gland cells of Mytilus galloprovincialis (Lam.). Marine Environmental Research, 16: 23-36.

Widdows, J. and Donkin, P. 1992. Mussels and environmental contaminants: bioaccumulation and physiological aspects. In: The Mussel Mytilus: Ecology, Physiology, Genetics and Culture, Gosling, E. (ed.). Elseviers Science Publishers, Amsterdam, pp. 399.

Widdows, J. 1985a. Physiological procedures. In: The Effects of Stress and Pollution on Marine Animals, B. L. Bayne, D. A. Brown, K. Burns, D. R.. Dixon, A. Ivanovici, D. R. Livingstone, D. M. Lowe, M. N. Moore, A. R. D. Stebbing and J. Widdows (eds.). Praegei Press, New York. pp. 161-178.

Widdows, J. 1985b. Physiological responses tc pollution. Marine Pollution Bulletin, 16: 129-134.

Williamson, P. D. 1980. Variables affecting body burdens of lead, zinc and cadmium in a road side population of the snail Cepaea hortensis Miiller. Oecologia (Ber.), 44: 213-220.

Wilson, J. and McMahon, R. 1981. Effects of high environmental copper concentration on the oxygen consumption, condition and shell morphology of natural populations of Mytilus edulis and Littorina rudis. Comparative Biochemistry and Physiology, 70C: 139-147.

Yap, C. K., Ismail, A., Tan, S. G. and Omar, H. 2002a. Correlations between speciation of Cd, Cu, Pb and Zn in sediment and their concentrations in total soft tissue of green-lipped mussel Perna viridis from the west coast of Peninsular Malaysia. Environment International, 28: 117-126.

Yap, C. K., Ismail, A. and Tan, S. G. 2002b. Laboratory studies on the tolerance and accumulation of cadmium, copper, lead and zinc by two different size groups of the green-lipped mussel Perna viridis (Linnaeus). Unpublished manuscript.

Zar, J.H., 1984. Biostatistical Analysis, 2nd Edition. Prentice/Hall, New Jersey. 718pp.

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