Malaysian Applied Biology Journal

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31-2-06

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Malays. Appl. Biol. (December 2002) 31(2): 47-55

ANALYSES OF INTRACELLULAR AND EXTRACELLULAR ISOZYMES OF GANODERMA ISOLATES FROM INFECTED OIL PALM AND COCONUT STUMPS

LATIFFAH, Z.1, TAN, S.G.2, ABDULLAH, F.2, HARIKRISHNA, K.1 and HO, Y.W.1*

1 Institute of Bioscience 2 Department of Biology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

ABSTRACT

Ganoderm isolates from infected oil palm and coconut stumps from different locations in Malaysia were compared using intracellular and extracellular isozyme analyses with a view to determine the genetic similarities and differences between the two groups of Ganoderm. Five intracellular enzymes, namely, alcohol dehydrogenase (ADH), acid phosphatase (ACP), alkaline phosphatase (AKP), hexokinase (HK) and glutamate oxaloacetate transaminase (GOT), were successfully typed using isoelectric focusing (IEF) at pH ranges of 3.5 - 9.5 and 5.5 - 8.5. The banding patterns of the five intracellular enzymes could not be used to differentiate between the Ganoderm isolates from oil palm and coconut stumps as variations occurred within and between the isolates of the two groups of Ganoderm. Three types of extracellular pectinase isozymes viz., polygalacturonase (PG), pectin esterase (PE) and pectin lyase (PL), were detected using polyacrylamide gel electrophoresis (PAGE). The pectinase isozyme patterns also did not produce specific patterns that could differentiate between the two groups of Ganoderm. Cluster analysis of the pectinase isozymes showed that the oil palm and coconut isolates were clustered together, indicating a close relationship.


ABSTRAK

Kajian perbandingan menggunakan analisis isozim intrasel and ekstrasel isolat-isolat Ganoderm pokok kelapa sawit yang dijangkiti dan tunggul kelapa dari beberapa kawasan di Malaysia telah dijalankan untuk melihat persamaan dan perbezaan dari segi genetik kedua-dua kumpulan Ganoderm tersebut. Lima enzim intrasel iaitu alkohol dehidrogenase ADH), acid phosphatase (ACP), alkaline phosphatase (AKP), hexokinase (HK) and glutamat oxaloasetat transaminase GOT) telah berjaya di perolehi menggunakan teknik 'isoelektrik focusing' (IEF) pada julat pH 3.5 - 9.5 dan 5.5 -*.5. Corak jalur kelima-lima enzim tersebut tidak dapat membezakan isolat-isolat Ganoderm kelapa sawit dan tunggul selapa disebabkan terdapatnya variasi pada corak jalur yang dihasilkan. Tiga jenis enzim pektinase dapat di kesan iaitu poligalakturonase (PG), pectin esterase (PE) and pectin liase (PL). Corak jalur isozim pektinase juga tidak menghasilkan sebarang jalur yang spesifik yang dapat membezakan di antara isolat Ganoderm kelapa sawit dan tunggul kelapa. Daripada analisa kluster isozim pektinase didapati isolat-isolat Ganoderm kelapa sawit dan tunggul kelapa berada dalam kelompok yang sama di mana menunjukkan kedua-dua kumpulan Ganoderm tersebut mempunyai pertalian yang rapat.

Key words: Ganoderm, oil palm, coconut, isozymes, isoelectric focusing

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