Malaysian Applied Biology Journal

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Malays. Appl. Bwl. (December 2002) 31(2): 9-18



1Suratthani Inland Fisheries Development Centre, Phunphin, Suratthani 84130, Thailand
2 Department of Biology, Faculty of Science and Environmental Studies, University Putra Malaysia, 43400 UPM, Serdang Selangor, Malaysia
3 Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
4National Aquaculture Genetics Research Institute, Klong Ha, Klong Loung, Pathumthani 12120, Thailand


Allozyme markers have proved to be very powerful to study genetic variation at interspecific level. Horizontal starch gel electrophoresis have been applied to differentiate the bagrids and tachysurids: Mystus nemurus, M. cavasius, M. gulio, Tachysurus caelatus and T. truncatus. The samples were collected from the Tapee River in the southern part of Thailand. Four tissues, heart, liver, kidney and muscle, from individual fish were analysed at 22 enzymatic loci. Eleven loci (52.38%) were found to be polymorphic (at p<0.05) namely Acp*. Aat-1*. Est*. Gpi-1*. Gpi-2*. Idh*. Me*. Pgm*. 6Pgd*. Sod* and Hb*. The percentage of polymorphic loci ranged from 14.3% in T. caelatus to 38.1% in M. nemurus. The highest mean heterozygosity was found in T. truncatus population (0.121 ±0.048) and the lowest in M. cavasius (0.026±0.014). The genetic distances among Mystus species ranged from 0.167 to 0.364 while the value oi 0.359 was found between the two species of Tachysurus. The genetic distance between Mystus and Tachysurus ranged from 0.693 to 0.878 indicating that both genera are rather distantly related to one another.


Penanda alozim telah terbukti berkesan untuk mengkaji variasi genetik pada paras interspesifik. Elektroforesis gel kanji horizontal telah diguna untuk membezakan bagrid dan tachysurid : Mystus nemurus, M. cavasius, M. gulio, Tachysurus caelatus dan T. truncatus. Sampel telah dikumpul dari Sungai Tapee di selatan Thailand. Empat tisu, jantung, hati, ginjal dan otot daripada individu ikan dianalisis pada 22 lokus enzim. Sebelas lokus (52.38%) didapati polimorfik (p<0.05) iaitu Acp*, Aat-1*, Est*, Gpi-1*, Gpi-2*, Idh*, Me*, Pgm*, 6Pgd*, Sod* and Hb*. Peratus lokus polimorfik menjulat daripada 14.3% dalam T caelatus ke 38.1% dalam M. nemurus. Min heterozigositi tertinggi telah didapati dalam populasi T.truncatus (0.121 ±0.048) dan terendah dalam M. cavasius (0.026±0.014). Jarak genetik dikalangan spesies Mystus menjulat daripada 0.167 ke 0.364 sementara nilai 0.359 telah dijumpai dalam kedua-dua spesies Tachysurus. Jarak genetik diantara Mystus dan Tachysurus menjulat daripada 0.693 ke 0.878, ini menunjukkan bahawa kedua-dua genera mempunyai pertalian yang jauh diantara satu sama lain.

Key words: Genetic variation, Mystus, Tachysurus, catfish, electrophoresis, allozyme


Bank, F. H. V. D., J. P. Grobler and H. H. D. Preez. 1992. A comparative biochemical genetic study of three populations of domesticated and wild African catfish (Clarias gariepinus). Comp. Biochem. Physiol. 101B(3): 387-390.

Carmichael, G. J., M. E. Schmidt and D. C. Morizot. 1992. Electrophoretic identification of genetic markers in channel catfish and blue catfish by use of low-risk tissues. Transaction of the American Fisheries Society. 121: 26-31.

Daud, S. K., I. Patimah and A. Kijima. 1989. Genetic variability and relationships among four groups of freshwater catfish. Malays. Appl. Biol. 18(1): 23-31.

Dodson, J. J., F. Colombani and P. K. L. Ng. 1993. Phylogeographic structure in mitochondrial DNA of a South-east Asian freshwater fish, Hemibagrus nemurus (Siluroidei; Bagridae) and Pleistocene sea-level changes on the Sunda shelf. Molecular Ecology. 4, 331-346.

Ferguson, A. 1984. Manual of electrophoretic techniques and analysis. Zoology Department, The Queen's University. 10 pp.

Grobler, J. P. and F. H. V. D. Bank. 1994. Allozyme polymorphism and phenotypic variation in African catfish (Clarias gariepinus). Comp. Biochem. Physiol. 107B: 111-116.

Ismail, P., S. K. Daud. and A. Kijima. 1989. Genetic control of isozymes in the four catfish species. Malays. Appl. Biol. 18: 33-37.

Leesanga, S., S. S. Siraj, S. K. Daud, S. G. Tan, P. K. Sodsuk and S. Sodsuk. 2000. Biochemical polymorphism in yellow catfish, Mystus nemurus (Cuv. & Val.), from Thailand. Biochemical Genetics 38: 77-85.

Mohsin, B. K. M. and M. A. Ambak. 1983. Freshwater fishes of Peninsular Malaysia. Faculty of Fisheries and Marine Science, Universiti Pertanian Malaysia, Selangor, Malaysia. 284 pp.

Nei, M. 1976. Mathematical models of speciation and genetic distance. In S. Karlin and E. Nevo (eds.) Population Genetics and Ecology. Academic Press, New York. 723-765 pp.

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small of individuals. Genetics 47, 253-259.

Nei, M. 1988. Genetic distance and molecular phylogeny. In Population genetics and fisheries management (N. Ryman & F. M. Utter, eds.), University of Washington Press, pp. 193-224.

Mevo, E. and C. R. Shaw. 1978. Genetic variation in the subterranean mammal, Spalax ehnenbergi. Biochemical Genetic 1, 235-241.

Richardson, B. J. 1986. Allozyme electrophoresis. Academic Press, Sydney.

Shaklee, J. B., F. W. Allendorf, D. C. Morizot and G. S. Whitt. 1990. Gene nomenclature for protein coding loci in fish. Transactions of The American Fisheries Society 119: 2-15.

Shaw, C. R. and R. Prasad. 1970. Starch gel electrophoresis of enzymes - A compilation of recipes. Biochemical Genetics 4: 297-320

Siraj, S. S., S. K. Daud, A. Othman and S. G. Tan. 1998. Population genetic structure of Baung, Mystus nemurus (C & V), in Malaysia. Malaysian Applied Biology 27: 77-82.

Siraj, S. S., S. Seki and N. Taniguchi. 1993. Genetic divergence and variability in Puntius spp. Japan Aquaculture Society 41(4): 469-474.

Smith, H. M. 1945. The freshwater fish of Siam or Thailand. United State Government Printing Office, Washington. 382-387pp

Swofford, D. L. and R. B. Selander. 1989. BIOSYS-1: A computer program for the analysis of allele variation in population genetics and biochemical systematics. Release 1.7. Illinois Natural History Survey, Champaign, Illinois, USA. 43 pp.

Taniguchi, N. and K. Suguma. 1990. Genetic variation and population structure of red sea bream in the coastal waters of Japan and the East China Sea. Nippon Suisan Gakkaishi (abstract) 56: 1069 - 1077.

Teugels, G. G., R. Guyomard and M. Legendre. 1992. Enzymatic variation in African clariid catfishes. Journal of Fish Biology 40: 87-96.

Ward, R. D. and P. M. Grewe. 1995. Appraisal of molecular genetic techniques in fisheries. In: Carvalho, G. R. and Pitcher (eds.), Molecular genetics in fisheries. Chapman and Hall. London. 29-54 on.

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