Malaysian Applied Biology Journal

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26-1-01

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Malays. App. Biol. (1997) 26(1): 1-5

EFFECTS OF SODIUM CHLORIDE ON CELLULASE PRODUCTION BY ASPERGILLUS TERREUS SUK-1

WAN MOHTAR, W.Y1*., OTHMAN, O.2, J. KADER1, SURESH, S1. and SHREE MANO, J1.

1 Department of Microbiology 2 Department of Biochemistry Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia

ABSTRACT

Batch culture studies of Aspergillus terreus SUK-1 were conducted to evaluate the effect of sodium chloride (NaCl) on the production of cellulase. Results showed that the presence of NaCl (0.05%, 0.10% and 0.50% w/v) in the medium enhancec the production of cellulase. The maximum productivity of CMCase obtained in experiments with 0.05%, 0.10% and 0.50% (w/v) NaCl concentrations were 10.83 x 10"3 lU/ml/hr, 8.33 x 10-1 lU/ml/hr and 7.50 x 10"3 lU/ml/hr respectively. Ir comparison, the maximum CMCase activity in experiments without NaCl was 4.17 x 10"1 lU/ml/hr. The concentration ol reducing sugar was found to increase in the experiments using NaCl, compared to experiments without NaCl. The maximurr reducing sugar obtained in experiments with 0.05%, 0.10% and 0.50% w/v NaCl were 14.40 x 10"' umol/ml 17.80 x 10"' umol/ml and 21.10 x 10"' umol/mi respectively, whereas the maximum reducing sugar in experiment without NaCl was only 5.0 x 10"' umol/ml. Visual observation of the sample at NaCl concentration of 1.00% (w/v) in the medium showec the highest intensity of colour but the highest CMCase activity was low.


ABSTRAK

Kajian berdasarkan kultur kelompok telah digunakan untuk mengkaji kesan natrium klorida (NaCl) ke atas penghasilar selulase oleh Aspergillus terreus SUK-1. Hasil kajian menunjukkan kehadiran NaCl (0.05%, 0.10% dan 0.50% w/v) dapal meningkatkan produktiviti selulase berbanding dengan eksperimen tanpa NaCl. Produktiviti CMCase bagi eksperimen-eksperimen yang menggunakan 0.05%, 0.10% dan 0.50% (w/v) NaCl masing-masing adalah 10.83 x 10"3 lU/ml/jam 8.33 x 10'3 lU/ml/jam dan 7.50 x 10"3 lU/ml/jam. Sementara nilai maksimum aktiviti CMCase bagi eksperimen tanpa NaCl ialah 4.17 x 10"3 lU/ml/jam. Kandungan gula penurun meningkat dalam eksperimen-eksperimen yang menggunakan NaCl berbanding dengan eksperimen tanpa NaCl. Nilai maksimum gula penurun dalam eksperimen yang dijalankan dengar 0.05%, 0.10% dan 0.50% (w/v) NaCl masing-masing ialah 14.40 x 10"' umol/ml, 17.80 x 10"' umol/ml dan 21.10 x 10"' umol/ml sementara nilai maksimum gula penurun bagi eksperimen tanpa NaCl ialah 5.0 x 10"' umol/ml. Keamatan warns yang paling tinggi dicerap dalam eksperimen yang menggunakan 1.00% (w/v) NaCl, tetapi aktiviti CMCase didapati rendah.

Key words: Batch culture, cellulase, sodium chloride, Aspergillus terreus SUK-1

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