Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

26-1-06

E-mail Print PDF

Malays. Appl. Bial. (1997) 26(1): 33-38

CYTOGENETICS OF INTERSPECIFIC HYBRIDS AND BACKCROSS PROGENIES FROM ORYZA SATIVA X O. MINUTA CROSSES

MARIAM, A.L.1, ZAKRI, A.H1., MAHANI, M.C.1* and NORMAH, M.N.2

1 School of Science and Technology, Universiti Malaysia Sabah, Locked Bag No 2073, 88999, Kota Kinabalu, Sabah, Malaysia
2 Department of Genetics, Department of Botany, Faculty of Life Sciences, Universiti Kebangsaan Malaysia,
43600 Bangi, Selangor DE, Malaysia

ABSTRACT

Somatic chromosome number and meiotic chromosome pairing were examined in Ft hybrids and BC progenies from Oryza saliva (AA) x O. minuta (BBCC) crosses. All hybrids were triploid with 36 chromosomes.The hybrids showed irregulai meiosis with mostly univalents. The mean meiotic pairing obtained was 29.31(16-36)Is + 3.32(0-10)IIs + 0.016(0-l)IIIs 4 0.002(0-l)IVs with 3.46(0-13) chiasmata per cell. The chromosome numbers of 17 BC, plants varied from 44 to 48 and from 24 to 37 in the BC2 plants. The mean bivalents in the BC,s varied from 9.83 (7-14) up to 13.90 (11-19). In the BC. plants, except the 24-chromosome BC2, the means of bivalents were from 8.58 (6-12) to 11.18 (8-17). Majority of the bivalents observed in both BC, and BC2 were ring-shaped. For the 24-BC.,, the meiosis was more regular. Of the 60 PMCs examined, 60% showed 12IIs, 23.3% showed 1 Ills + 2Is, 13.3% showed 1011s + 4Is and 3.3% showed 9IIs + 6Is.


ABSTRAK

Kromosom somatik dan perpasangan kromosom meiosis telah dikaji pada hibrid F, dan progeni kacukbalik daripada kacukan Oryza saliva (AA) x O. minuta (BBCC). Kesemua hibrid adalah triploid dengan bilangan kromosom 36, dan menunjukkan meiosis yang tidak regular. Purata dan julat perpasangan kromosom semasa meiosis adalah 29.31 (16-36)1 + 3.32 (0-10)11 + 0.016 (0-1)111 + 0.002 (0-1 )IV, dengan 3.46(0-13) kiasma setiap sel. Bilangan kromosom 17 tumbuhan kacukbalik-1 adalah di antara 44 dan 48, dan di antara 24 dan 37 untuk tumbuhan kacukbalik-2. Purata bivalen dalam kacukbalik-1 adalah di antara 9.83(7-14) dan 13.90(11-19). Dalam tumbuhan kacukbalik-2 (kecuali untuk tumbuhan kacukbalik-2 yang mempunyai 24 kromosom), bilangan purata bivalen adalah di antara 8.58(6-12) dan 11.18(8-17). Kebanyakan bivalen yang dicerap dalam kacukbalik-I dan kacukbalik-2 adalah berbentuk cincin. Untuk tumbuhan kacukbalik-2 yang mempunyai 24 kromosom, meiosis adalah lebih regular. Daripada 60 PMC yang dicerap, 60% menunjukkan 1211, 23.3% menunjukkan 1 III + 21, 13.3% menunjukkan 1011 + 41 dan 3.3% menunjukkan 911 + 61.

Key words: Meiotic pairing, Oryza, wide hybridization

REFERRENCES

Alexander, M.P. 1980. A versatile stain for pollen, fungi, yeast and bacteria. Stain Technology, 55: 13-18.

Amante-Bordeous, A.D., Sitch, L.A., Nelson, R.J., Dalmacio, R.D., Oliva, N.P., Aswidinnoor, H. and Leung, H. 1992. Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theoretical and Applied Genetics, 84: 345-354.

Jena, K.K. and Khush, G.S. 1984. Embryo rescue of interspecific hybrids and its scope in rice improvement. Rice Genetics Newsletter, 1: 133-134.

Jena, K.K. and Khush G.S. 1990. Introgression ol genes from Oryza officinalis Well ex Watt tc cultivated rice, O. sativa L. Theoretical ana Applied Genetics, 80: 737-745.

Katayama, T. 1966. Cytogenetical studies on the genus Oryza. 2. Chromosome pairing in the interspecific hybrid with the ABC genomes. Japan Journal of Genetics, 41: 309-316.

Katayama, T. 1967. Cytogenetical studies on the genus Oryza. IV. Cytological studies on the first backcross generation of the (AxBC)xA and (AxCD)xA genomes. Japan Journal of Genetics, 42: 169-174.

Kihara, H., Nezu, M. and Katayama, C. 1961. Genome analysis in the genus Oryza. ll-Annual Report National Institute of Genetics Japan II (1960): 40-41.

Li, H.W., Chen, C.C., Wang, T.S. and Wuu, K.D. 1963. Cytogenetical studies of Oryza sativa L. and its related species. 4. Interspecies crosses involving Oryza australiensis with O. sativa and O. minuta. Botanical Bulletin Academia Sinica, 4: 65-74.

Mariam, A.L., Zakri, A.H., Mahani, M.C. and Normah, M.N. 1996. Interspecific hybridization of cultivated rice Oryza sativa L. with the wild rice O. minuta Presl. Theoretical and Applied Genetics, 93: 664-671.

Multani, D.S., Jena, K.K., Brar, D.S., De los Reyos, B.C., Angeles, E.R. and Khush, G.S. 1994. Development of monosomic alien addition lines and introgression of genes from O. australiensis Domin. to cultivated rice O. sativa L. Theoretical and Applied Genetics, 88: 102-109.

Nezu, M., Katayama, T.C. and Kihara, H. 1960. Genetic study of the genus Oryza. I. Crossability and chromosomal affinity among 17 species. SeikenZiho, 11: 1-11.

Snow, R. 1963. Alcoholic hydrochloric acid-cannine as a stain for chromosomes in squash preparations. Stain Technology, 38: 9-13.

Vaughan, D.A. 1989. Collection, conservation and potential use of the wild relatives of rice in Asia and Australia. In: Mujeeb-Kaji, A. and Sitch, L.A. (eds.) Reviews in Plant Biotechnology, 1985-1988. International Maize and Wheat Improvement Center (CIMMYT), Mexico, and International Rice Research Institute, Manila, Philippines, pp. 179-190.
 

Latest MABJ Issue

Vol 48(3) June 2019

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Myjurnal, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.