Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2006) 35(1): 57–62



1 Department of Food Service and Management, Faculty of Food Science and Technology 2 Department of Microbiology, 3 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
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The tolerance of Bifidobacterium pseudocatenulatum to simulated gastric pH was investigated. Twelve B. pseudocatenulatum strains isolated from breast-fed infant faeces were exposed to HCl acid adjusted to pH 1.0, 2.0 and 3.0 for 3 hr. At pH 1.0, three strains, D19, F91 and G4 survived 1 hr exposure, with a decline in viability of about 4-5 log cfu ml-1. After 2 hr, no viable cells could be detected for all isolates. At pH 2.0, strains G4, D19, C20 and A50 were able to endure and maintain high number of viable cells following 3 hr exposure. The other strains showed progressive reduction in viability during 2 and 3 hr incubation. Strain F91, which survived pH 1.0 for 1 hr could not tolerate pH 2.0 after 2 hr. At pH 3.0, all strains exhibited high endurance with more than 5 log cfu ml-1 after 3 hr. High survival rate was observed at pH 6.5 (control), with little or no decrease in cell count. Among all the strains tested, B. pseudocatenulatum G4 and D19 demonstrated the best tolerance to low pH, thus suggesting potential as probiotic candidate.


Ketahanan bakteria Bifidobacterium pseudocatenulatum terhadap pH rendah, seumpama keadaan asidik perut telah dikaji. Dua belas strain B. pseudocatenulatum yang dipencil dari najis bayi yang bersusu ibu telah didedahkan kepada asid HCl pada pH 1.0, 2.0 and 3.0 selama 3 jam. Pada pH 1.0, hanya strain D19, F91 dan G4 berupaya hidup selepas 1 jam pendedahan, tetapi bilangannya menurun sebanyak 4-5 log cfu ml-1. Manakala selepas 2 jam, kesemua strain itu tidak dapat dipencilkan. Pada pH 2.0, strain G4, D19, C20 dan A50 berupaya untuk bertahan dan mengekalkan sel berdaya hidup yang tinggi selama 3 jam. Bilangan cfu ml-1 bagi strain lain menurun selepas 2 hingga 3 jam pendedahan. Walaupun isolat F91 berupaya bertahan keadaan berasid pH 1.0 selama 1 jam, bilangan cfu ml-1 menurun dengan drastik apabila didedahkan selama 2 jam pada pH 2.0. Pada pendedahan selama 3 jam di pH 3.0, semua strain mempunyai ketahanan yang baik, di mana bilangan cfu ml-1 adalah melebihi daripada 5 log cfu ml-1. Manakala pada pH 6.5, semua strain menunjukkan ketahanan yang baik. Daripada kajian ini, dapat disimpulkan bahawa B. pseudocatenulatum G4 dan D19 memaparkan ketahanan yang paling tinggi terhadap keadaan asidik berbanding dengan strain lain. Dengan itu, kedua-dua strain ini boleh disyorkan sebagai calon probiotik.

Key words: Survival, bifidobacteria, Bifidobacterium pseudocatenulatum, low pH, probiotics


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