Malaysian Applied Biology Journal

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37-2-06

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Malays. Appl. Biol. (2008) 37(2): 35–40

THE CONCENTRATIONS OF HEAVY METALS IN DIFFERENT TISSUES OF HORSESHOE CRABS COLLECTED FROM INTERTIDAL AREAS OF JOHOR, PENINSULAR MALAYSIA

YAP, C.K.1*, MOHD HAFETZ, A.1 and TAN, S.G.2

1 Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia.
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it or This e-mail address is being protected from spambots. You need JavaScript enabled to view it

ABSTRACT

Horseshoe crabs (Tachypleus gigas) were collected from the intertidal areas of Tampok, Pontian Besar and Kg. Pasir Puteh in Johore, in May 2007. They were dissected into five parts namely carapace, muscle (from foot), operculum, gills and fillet. For each site, the different tissues from the animals were pooled and triplicates of each category were determined for the concentrations of Cd, Cu, Fe, Pb and Zn. Based on the metal distribution patterns in the T. gigas from all the sites, four distinctive patterns were found. It showed that different tissues of T. gigas accumulated different levels of metals. The gills accumulated the highest concentrations of Fe, Cu, Pb and Cd while the carapace accumulated the lowest concentrations of Cd, Cu, Fe, Pb and Zn. Except for Cd, all of the metal levels in the fillet were similar. Finally, the elevated concentrations of Cd and Zn in the fillet indicated anthropogenic-induced bioavailabilities of Cd and Zn. These results are useful baseline data for future comparisons.

Key words: Horseshoe crab (Tachypleus gigas), heavy metals

REFERRENCES

Botton, M.L. 2000. Toxicity of cadmium and mercury to horseshoe crab (Limulus Polyphemus), embryos and larvae. Bulletin of Environmental Contamination and Toxicology, 64: 137–143.

Botton, M.L., Johnson, K. & Helleby, L. 1998. Effects of copper and zinc on embryos and larvae of the horseshoe crab, Limulus Polyphemus. Archives of Environmental Contamination and Toxicology, 64: 25–32.

Burger, J. 1992. Trace element levels in Pine Snake hatchlings: tissue and temporal differences. Archives of Environmental Contamination and Toxicology, 22: 209–213.

Burger, J. 1993. Metals in avian feathers: bioindicators of environmental pollution. Review of Environmental Toxicology, 5: 203– 311.

Burger, J. 1994. Heavy metals in avian eggshells: another excretion method. Journal of Toxicology and Environmental Health, 41: 207–220.

Burger, J. 1997. Heavy metals in the eggs and muscle of horseshoe crabs (Limulus poly- phemus) from Delaware Bay. Environmental Monitoring and Assessment, 46: 279–287.

Burger, J., Dixon, C., Shukla, T., Tsipoura, N., Jensen, H., Fitzgerald, M., Ramos, R. & Gochfeld, M. 2003. Metals in Horseshoe Crabs from Delaware Bay. Archives of Environmental Contamination and Toxicology, 44: 36–42.

Castro, G. & Myers, J.P. 1993. Shorebird predation on eggs of horseshoe crabs during spring stopover on Delaware Bay. Auk, 110: 927–930.

Castro, G., Myers, J.P. & Place, A.R. 1989. Assimilation efficiency of sanderlings (Calidris alba) feeding on horseshoe crab eggs. Physiological Zoology, 62: 716–731.

Chatterji, A., Mishra, J.K. & Parulekar, A.H. 1992. Feeding behaviour and food selection in the horseshoe crab, Tachypleus gigas (Muller). Hydrobiologia, 246: 41-18.

Kungsuwan, A., Nagashima, Y., Noguchi, T., Shida, Y., Suvapeepan, S., Suwansakornkul, P. & Hashimoto, K. 1987. Tetrodotoxin in the horseshoe crab, Carcinoscorpius rotundicauda inhabiting Thailand. Nippon Suisan Gakkaishi, 53: 261-266.

Mohan, S., Doral, D.T., Srimal, S., Bachhawat, B.K. & Das, M.K. 1984. Circular dichroism studies on carcinoscorpin, the sialic acid binding lectin of horseshoe crab, Carcinoscorpius rotundacauda. Indian Journal of Biochemical Biophysics, 21: 151-154.

Rainbow, P.S. 1996. Heavy metals in aquatic in vertebrates. In: Environmental Contaminants in Wildlife: Interpreting Tissue Concentrations. W.N. Beyer, G.H. Heinz and A.W. Redmon- Norwood (Eds.). Lewis Pubblicaton, Boca Raton, FL, pp. 405–425.

Sekiguchi, K., Nakamura, K., Sen, T.K. & Sugita, H. 1976. Morphological variation and distribution of a horseshoe crab, Tachypleus gigas, from the Bay of Bengal and Gulf of Siam. Proceedings of the Japanese Society of Systematic Zoology, 12: 13-20.

Shuster, C.N. Jr. 1985. Introductory remarks on the distribution and abundance of the American horseshoe crab, Limulus poly- phemus, spawning in the Chesapeake Bay area. In: Proceedings from the Chesapeake Bay Symposium. V. Chase (Ed.). Baltimore, MD, pp. 34-38.

Tsipoura, N. & Burger, J. 1999. Shorebird diet during spring migration stop-over on Delaware Bay. Condor, 101: 35–644.

Yap, C.K., Ismail, A. & Tan, S.G. 2003a. Cd and Zn in the straits of Malacca and intertidal sediments of the west coast of Peninsular Malaysia. Marine Pollution Bulletin, 46: 1348- 1353.

Yap C.K., Ismail, A., Tan, S.G. & Abdul Rahim, I. 2003b. Can the shell of the green-lipped mussel Perna viridis from the west coast of Peninsular Malaysia be a potential biomonitoring material for Cd, Pb and Zn? Estuarine and Coastal Shelf Science, 57: 623- 630.

Yap, C.K., Tan, S.G., Ismail, A. & Omar, H. 2004a. Allozyme polymorphisms and heavy metal levels in the green-lipped mussel Perna viridis (Linnaeus) collected from contaminated and uncontaminated sites in Malaysia. Environment International, 30: 39-46.

Yap, C.K., Ismail, A, Tan, S.G. & Rahim Ismail, A. 2004b. The impact of anthropogenic activities on heavy metal (Cd, Cu, Pb and Zn) pollution: Comparison of the metal levels in green-lipped mussel Perna viridis (Linnaeus) and in the sediment from a high activity site at Kg. Pasir Puteh and a relatively low activity site at Pasir Panjang. Pertanika Journal of Tropical Agricultural Science, 27(1): 73-78.

Yap, C.K., Ismail, A., Edward Berandah, F., Tan, S.G. & Siraj, S.S. 2006. Use of different soft tissues of Perna viridis as biomonitors of bioavailability and contamination by heavy metals (Cd, Cu, Fe, Pb, Ni and Zn) in a semi- enclosed intertidal water, the Johore Straits. Toxicological and Environmental Chemistry, 88(4): 683 - 695.
 

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