Malaysian Applied Biology Journal

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36-1-04

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Malays. Appl. Biol. (2007) 36(1): 23–29

GENETIC CHARACTERIZATION OF THE TWO COLOUR-TYPE OF KELAH

SIRAJ, S.S., ESA, Y.B., KEONG, B.P. and DAUD, S.K.

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
*E-mail : This e-mail address is being protected from spambots. You need JavaScript enabled to view it ,my

ABSTRACT

Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three individuals of the kelah were sampled from Sia River of Pahang and Kampung Esok River of Negeri Sembilan (silver- bronze) and Nenggiri River of Kelantan (reddish). Twelve RAPD primers generated a total of 226 scorable loci with 100% polymorphism across the sixty-three individuals. The RAPD banding patterns and sizes ranged from 4 to 17 and from 100bp to 1500bp, respectively. The intra-population UPGMA dendrogram produced two major clusters, with the Nenggiri River (Kelantan) samples formed a subcluster in both major clusters dominated by the Pahang samples (Cluster 1) and N. Sembilan (Cluster 2) samples, respectively. The inter-population UPGMA showed that the Kelantan samples were genetically closer to the N. Sembilan samples than to the Pahang samples. Thus, the results of this study did not support the hypothesis that the two colour-types of T. tambroides were genetically distinct from each other.


ABSTRAK

Penanda ‘Randomly Amplified Polymorphic DNA (RAPD)’ telah digunakan untuk melihat hubungan genetik di kalangan tiga populasi ikan kelah (Tor tambroides) yang mempunyai dua jenis warna berbeza (perak-gangsa dan kemerahan). Enam puluh tiga individu kelah telah disampel dari Sungai Sia, Pahang dan Sungai Kampung Esok, Negeri Sembilan (jenis warna perak-gangsa) dan Sungai Nenggiri, Kelantan (jenis warna kemerahan). Dua belas “primer” RAPD yang telah dipilih menghasilkan sejumlah 226 lokus yang 100% polimorfik daripada enam puluh tiga individu tersebut. Corak dan saiz lokus RAPD yang terhasil daripada ketiga-tiga populasi berjulat daripada 4 hingga 17 lokus, dan daripada 100bp hingga 1500bp, masing-masing. Keputusan dendrogram UPGMA intra-populasi menghasilkan dua kelompok utama; sampel-sampel dari Sungai Nenggiri (Kelantan) membentuk subkelompok di dalam kedua-dua kelompok utama yang didominasi oleh masing-masing sampel-sampel dari Pahang (Kelompok 1) dan N. Sembilan (Kelompok 2). Keputusan dendrogram UPGMA untuk inter-populasi menunjukkan sampel-sampel dari Kelantan adalah lebih dekat secara genetik dengan sampel-sampel dari N. Sembilan berbanding sampel-sampel dari Pahang. Justeru, keputusan daripada kajian ini tidak menyokong hipotesis bahawa kedua-dua jenis warna T. tambroides adalah berbeza secara genetik di antara satu sama lain.

Key words: Freshwater fish, Tor tambroides, colour-type, RAPD, genetic marker

REFERRENCES

Armstrong, J.S., Gibbs, A.J., Peakall, R. and Weiller, G. 1994. The RAPDistance Package, version 1.04. Australian National University, Canberra.

Badarkci, F. and Skibinski, D.O.F. 1994. Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 73: 117-123.

Bartish, I.V., Garkava, L.P., Rumpunen, K. and Nybom, H. 2000. Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theori- tical and Applied Genetics 101: 554-561.

Chang, W.S. 2000. Features of Indigenouus Fish Species Having Potentials for Aquaculture. Inland Fisheries Division of Department of Agriculture, Sarawak, pp 2-7.

Dahle, G., Rahman, M. and Erikksen, A.G. 1997. RAPD fingerprinting used for discriminating among three populations of Hilsa shad (Tenualosa ilisha). Fisheries Research 32: 263- 269.

D’Amato, M.E. and Corach, D. 1996. Genetic diversity of populations of the freshwater shrimp Macrobrachium borelli (Caridae: Palaemonidae). Journal of Crustacean Biology. 16(4): 650-655.

Dodson, J.J., Colombani, F. and Ng, P.K.L. 1995. Phylogeographic structure in mitochondrial DNA of a Southeast Asian freshwater fish, Hemibagrus nemurus (Siluroidei; Bagridae) and Pleistocene sea-level changes on the Sunda shelf. Molecular Ecology 4: 331-346.

Eddy, S.P.T. 1997. Angling for kelah. Rod and Line Magazine. November 1997, pp 68-74.

Esa, Y.B., Khairul Adha, A. Rahim., Kamaruddin, K.R., Daud, S.K., Siraj, S.S. and Tan, S.G. 2006. Phylogenetic relationships among three freshwater fishes of the subtribe Tores (cyprinidae) in Malaysia inferred from sequencing of a cytochrome c oxidase I segment. In: The 1st International Symposium on the Mahseers, Kuala Lumpur, 28-29 March 2006.

Hadrys, H., Balick, M. and Sshierwater, B. 1992. Application of Random Amplified Poly- morphic DNA (RAPD) in molecular ecology. Molecular Ecology Notes 1: 55-63.

Harris, S.A. 1999. RAPDs in systematics - A useful methodology? In Advances in Molecular Systematics, ed. P.M Hollingsworth, R.M. Bateman and R.J Gornall, pp 211-28. London: Taylor & Francis.

Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, W.O., Sala, F., van de Wiel, C., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P., Buiatti, M., Maestri, E., Malcevschii, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. and Karp, A. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by network of European Laboratories. Molecular Breeding 3: 381-390.

Jong, M.Y. and Gye, W.K. 2001. Random amplified polymorphic DNA – polymerase chain reaction analysis of two different populations of cultured Korean catfish Silurus asotus. Journal of Bioscience. 26(5): 641-647.

Koh, T.L., Khoo, G., Fan, L.O. and Phang, V.P.E. 1999. Genetic diversity among wild forms and cultivated varieties of discus (Symphysodon spp.) as revealed by Random Amplified Polymorphic DNA (RAPD) fingerprinting. Aquaculture 173: 485-497.

Kottelat, M., Whitten, A.J., Kartikasari, S.N. and Wirjoatmodjo, S. 1993. Freshwater fishes of Western Indonesia Sulawesi. Periplus Edition Ltd, Singapore.

Kumar, S.V., Saw, T.M., Tan, S.G., Quah, S.C. and Yusoff, K. 2003. Variation of Cultivated Mungbean and Wild Vigna as Revealed by Randomly Amplified Polymorphic DNA markers. Pertanika Journal of Tropical Agriculture Science 26(2): 123-129.

Lehmann, E.L. 1975. Nonparametric: Statistical Methods Based on Ranks. San Francisco: Holden-Day.

Mohsin, A.K.M. and Ambak, A.M. 1983. Freshwater fishes of Peninsular Malaysia, Malaysia: Penerbit Universiti Pertanian Malaysia.

Nei, M and Li, W.H. 1979. Mathematical model for studying genetic variation in term of restriction endonucleases. Proceedings of the National Academy of Science 76: 5629-5273.

Ng, C.K. 2004. Kings of the rivers-mahseer in Malaysia and the region, pp 14-102. Selangor: Inter Sea Fishery (M) Sdn. Bhd.

Nguyen, T.T.T., Ingram, B., Sungan, S., Gooley, G., Sim, S.Y., Tinggi, D. and De Silva, S S. 2006. Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and Tor douronensis (Cyprinidae) cultured in Sarawak. Aqua- culture 253: 259-269.

Rainboth, W.J. 1996. Fishes of the Cambodian Mekong. FAO Species Identification Field Guide for Fishery Purposes. Food and Agriculture Organization, Rome.

Rohlf, F.J. 1989. NTSYS-PC numerical taxonomy and multivariate analysis system. Version 1.30. Exeter Software, New York.

Sneath, P.H.A. and Sokal, R.R. 1973. Numerical Taxonomy. In The principles and Practice of Numerical Classification, San Francisco: W.H. and Company.

Taggart, JB., Hyness, R.A., Prodohl, P.A. and Fergusan, A. 1992. A Simplified Protocol for Routine Total DNA Isolation from Salmonid Fishes. Journal of Fish Biology 40: 963-965.

Terumi, H. and Pedro, M.G. Jr. 2002. RAPD markers indicate the occurrence of structured populations in a migratory freshwater fish species. Genetics and. Molecular Biology, 26(1): 19-25.

Vrijenhoek, R. 1998. Conservation genetics of freshwater fish. Journal of Fish Biology. 53: 394 - 412.
 

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