Malaysian Applied Biology Journal

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42_2_03

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Malays. Appl. Biol. (2013) 42(2): 21–31

 

ISOLATION AND IDENTIFICATION OF Aphanomyces SPECIES

FROM NATURAL WATER BODIES AND FISH FARMS IN

SELANGOR, MALAYSIA



AFZALI, S.F.1, HASSAN, M.D.2*, ABDUL-RAHIM, A.M.1, SHARIFPOUR, I.3

and SABRI, J.1



1Aquatic Animal Health Unit, Faculty of Veterinary Medicine, Universiti Putra Malaysia,

43400 UPM, Serdang, Selangor D.E, Malaysia.

2Marine Biotechnology Laboratory, Institute of Bioscience Universiti Putra Malaysia,

43400 UPM, Serdang, Selangor D.E, Malaysia.

3Iranian Fisheries Research Organization (IFRO), Tehran, Iran

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Tel: 603-8947 2111

Fax: +603-8947 2101



ABSTRACT



This study was conducted to isolate and identify fresh water fungi species from the Malaysian natural water bodies and fish farms and to examine the pathogenicity of the isolates as a confirmative identification tool for epizootic ulcerative syndrome (EUS) outbreak in Selangor state, Malaysia. For this aim, 165 water samples and 62 infected fish collected from 12 stations were tested in which 35 and 24 samples were found to be positive for fungi contamination and/or infection, respectively. The isolates were morphologically characterized; from 59 isolates, 32 were identified as Saprolegina, 21 as Achlya and 6 as Aphanomyces species. Experimental infection was carried out by intramuscularly injection of the Aphanomyces spp. Isolates to the Malaysian moonlight gourami (Trichogaster Microlepis), where no mortality and no signs of EUS were observed in the fish groups. Histopathology test also revealed no signs of damage in the skin, muscles and other tissues following infection with the isolates indicating that all the Aphanomyces isolates were non-pathogenic.

Key words: AphanomycesSaprolegniaAchlya, Epizootic Ulcerative Syndrome (EUS), histopathology



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