Malaysian Applied Biology Journal

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43_1_11

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Malays. Appl. Biol. (2014) 43(1): 97–105

 

INTERACTIVE EFFECT OF AMMONIUM AND NITRATE ON
THE NITROGEN UPTAKE AND BIOMASS PRODUCTION
BY Chlorella vulgaris IN DIFFERENT
ENVIRONMENTAL CONDITIONS

 

 

TAHERI, R. and SHARIATI, M.*



Department of Biology, Faculty of Science, University of Isfahan, Iran
*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

 


ABSTRACT

 


Microalgae assimilate different types of nitrogen by interacting in different areas. The interactive effect of uptake rate and biomass production of Chlorella vulgaris was investigated under summer and fall environmental conditions. C. vulgaris was cultivated in a media containing 1 mM NH4Cl, 0.5 mM NH4Cl + 0.5 mM KNO3, and 1 mM KNO3 as the nitrogen source. Nitrate and ammonium was more rapidly utilized when both were available. C. vulgaris preferred ammonium to nitrate in the fall condition, but both nitrate and ammonium were completely assimilated in the summer condition. The uptake rate of nitrate and ammonium in the summer condition was significantly higher (p < 0.01) than in the fall condition and the summer/fall ratio for nitrate uptake (290%) was significantly higher (p < 0.01) than for ammonium (120%). In all experiments, biomass production in the summer condition was significantly higher than fall condition (p < 0.01). The highest biomass was during the summer condition (5 g/l (f wt)) from the medium containing NO3- as the nitrogen source. C. vulgaris recorded faster growth in the NO3- and (NO3- + NH4+) media than in the NH4+ medium. The summer/fall ratio of biomass production was 235%, 300%, and 265% for the media containing NO3-, NH4+, and (NO3- + NH4+), respectively.

Key words: nitrate, ammonium, uptake rate, biomass production, Chlorella vulgaris



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