Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2014) 43(1): 97–105


BY Chlorella vulgaris IN DIFFERENT




Department of Biology, Faculty of Science, University of Isfahan, Iran
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Microalgae assimilate different types of nitrogen by interacting in different areas. The interactive effect of uptake rate and biomass production of Chlorella vulgaris was investigated under summer and fall environmental conditions. C. vulgaris was cultivated in a media containing 1 mM NH4Cl, 0.5 mM NH4Cl + 0.5 mM KNO3, and 1 mM KNO3 as the nitrogen source. Nitrate and ammonium was more rapidly utilized when both were available. C. vulgaris preferred ammonium to nitrate in the fall condition, but both nitrate and ammonium were completely assimilated in the summer condition. The uptake rate of nitrate and ammonium in the summer condition was significantly higher (p < 0.01) than in the fall condition and the summer/fall ratio for nitrate uptake (290%) was significantly higher (p < 0.01) than for ammonium (120%). In all experiments, biomass production in the summer condition was significantly higher than fall condition (p < 0.01). The highest biomass was during the summer condition (5 g/l (f wt)) from the medium containing NO3- as the nitrogen source. C. vulgaris recorded faster growth in the NO3- and (NO3- + NH4+) media than in the NH4+ medium. The summer/fall ratio of biomass production was 235%, 300%, and 265% for the media containing NO3-, NH4+, and (NO3- + NH4+), respectively.

Key words: nitrate, ammonium, uptake rate, biomass production, Chlorella vulgaris


Abraham, T. J., Ghosh S., Nagesh T. & Sasmal D. 2004. Distribution of bacteria involved in nitrogen and sulphur cycles in shrimp culture systems of West Bengal, India. Aquaculture. 239(1): 275-288.

Bauer, W. D. & Robinson J. B. 2002. Disruption of bacterial quorum sensing by other organisms. Current Opinion in Biotechnology. 13(3): 234-237.

Becker, E. W. 1993. Microalgae: biotechnology and microbiology 10. Cambridge University Press.

Chen, R., Li R., Deitz L., Liu Y., Stevenson R. J. & Liao W. 2012. Freshwater algal cultivation with animal waste for nutrient removal and biomass production. Biomass and Bioenergy. 39(128-138).

Chinnasamy, S., Bhatnagar A., Hunt R. W. & Das K. 2010. Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresource Technology. 101(9): 3097-3105.

Davis, E. M. & Wilcomb M. J. 1967. Enzymatic degradation and assimilation of condensed phosphates by green algae. Water Research. 1(5): 335-350.

Dortch, Q. 1990. The interaction between ammonium and nitrate uptake in phytoplankton. Marine ecology progress series. Oldendorf. 61(1): 183-201.

Eaton, A. D., Franson M. A. H., Association A. P. H., Association A. W. W. & Federation W. E. 2005. Standard methods for the examination of water & wastewater. American Public Health Association. pp 580-800.

Giangrande, A., Cavallo A. & Pierri C. 2007. Ammonium uptake of Cladophora Prolifera (Chlorophyta, Cladophorales) a candidate species for bioremediation of aquaculture wastes. Thalassia Salentina. 30: 107-116.

Gomez, P. I. & Gonzalez M. A. 2005. The effect of temperature and irradiance on the growth and carotenogenic capacity of seven strains of Dunaliella salina (Chlorophyta) cultivated under laboratory conditions. Biological Research. 38(2/3): 151.

Hadi, M., Shariati M. & Afsharzadeh S. 2008. Microalgal biotechnology: Carotenoid and glycerol production by the green algae Dunaliella isolated from the Gave-Khooni salt marsh, Iran. Biotechnology and Bioprocess Engineering. 13(5): 540-544.

Hii, Y., Soo C., Chuah T., Mohd-Azmi A. & Abol-Munafi A. 2011. Interactive effect of ammonium and nitrate of the nitrogen uptake by Nannochloropsis sp. Journal of Sustainability Science and Management. 6(1): 60-68.

Hosseini Tafreshi, A. & Shariati M. 2009. Dunaliella biotechnology: methods and applications. Journal of Applied Microbiology. 107(1): 14-35.

Jiménez, C., Capasso J. M., Edelstein C. L., Rivard C. J., Lucia S., Breusegem S., Berl T. & Segovia M. 2009. Different ways to die: cell death modes of the unicellular chlorophyte Dunaliella viridis exposed to various environmental stresses are mediated by the caspase-like activity DEVDase. Journal of Experimental Botany. 60(3): 815-828.

Kim, J., Lingaraju B. P., Rheaume R., Lee J.-Y. & Siddiqui K. F. 2010. Removal of Ammonia from Wastewater Effluent by Chlorella Vulgaris. Tsinghua Science & Technology. 15(4): 391-396.

Perez?Garcia, O., De?Bashan L. E., Hernandez J. P. & Bashan Y. 2010. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense1. Journal of Phycology. 46(4): 800-812.

Taiz, L. & Zeiger E. 2006. Plant physiology. Sunderland: Sinauer. pp 735-953.

Thakur, A. & Kumar H. 1999. Nitrate, ammonium, and phosphate uptake by the immobilized cells of Dunaliella salina. Bulletin of environmental contamination and toxicology. 62(1): 70-78.

Wang, L., Min M., Li Y., Chen P., Chen Y., Liu Y., Wang Y. & Ruan R. 2010. Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Applied Biochemistry and Biotechnology. 162(4):1-13.

Yan, D., Dai J. & Wu Q. 2013. Characterization of an ammonium transporter in the oleaginous alga Chlorella protothecoides. Applied Microbiology and Biotechnology. 97(2): 919-928.


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