44_4_07

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Malays. Appl. Biol. (2015) 44(4): 47-58

ANALYSIS OF BIOACTIVE PHYTOCHEMICAL COMPOUNDS OF TWO MEDICINAL PLANTS HORSETAIL Equisetum arvense AND Alchemila valgaris SEEDS BY USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND FOURIER-TRANSFORM INFRARED SPECTROSCOPY

HUDA JASIM ALTAMEME 1 , IMAD HADI HAMEED *1, NIDAA ADNAN ABU-SERAG2

1Department of Biology, Babylon University ,Hilla ,Iraq

2Department of Biology, College of Science, Babylon University, Hilla City, Iraq

*Corresponding author: Imad H. Phone number: 009647716150716,E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

ABSTRACT

Twenty five bioactive phytochemical compounds were identified in the methanolic extract of Equisetum arvense and Alchemila valgaris. The identification of phytochemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC/MS analysis of Equisetum arvense revealed the existence of the Cyclohexene, 4-isopropenyl-1-methoxymethyl, ?-D-Glucopyranoside, O-?-D-Glucopyranosyl, Paromomycin, 3,6,9,12-Tetraoxatetradecan-1-ol, Bicyclo[3.2.1] oct-6-ene-6,8-dimethanol, 1,7-dimethyl-4isopropyl, 2-Undecanone, 3-N,N-Dimethyllaurylammonio) propanesulfonate, d-Mannose, 3-O-Methyl-d-glucose, 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol, Benzaldehyde, 2-chloro-4-hydroxy-3, Cyclopropa[3,4]cyclopenta [1,2-a]naphthalene, Furo[2,3-b]quinoline,4,6,7-trimethoxy, 2(1H)-Phenanthrenone. GC/MS analysis of Alchemila valgaris revealed the existence of the Levoglucosenone, Spirost-8-en-11-one, 3-hydroxy, Ethanol, 2-(9-Octadecenyloxy), 2,7-Diphenyl-1,6dioxopyridazino[4,5:2,3]pyrrolo, Estra-1,3,5(10)-trien-17?-ol, Octadecanal,2-bromo, Ethyl 9,12,15-octadecatrienote, 3-Pyridinecarboxylic acid,2,7,10-tris(acetyloxy)-1, 5H-Cyclopropa[3,4]benz[1,2-e]azulen-5-one, Stigmastan-3,5-diene and Tocopherol. It contain chemical constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic. The FT-IR analysis of both Equisetum arvense and Althaea rosea seeds proved the presence of Alkenes, Aliphatic fluoro compounds, Alcohols, Ethers, Carboxlic acids, Esters, Nitro Compounds and Alkanes.

Keywords: Alchemila valgaris, Equisetum arvense, FT-IR, Gas chromatography-mass spectrometry, Phytochemicals.