Malays. Appl. Biol. (2009) 38(1): 71–79
GENETIC VARIABILITY AMONGST Fusariu m spp.IN THE SECTION LISEOLA FROM BAKANAE-INFECTED RICE IN MALAYSIA AND INDONESIA BY RAPD ANALYSIS
NUR AIN IZZATI, M.Z.1* and SALLEH, B.2
1Department of Biology, Faculty of Science, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
2School of Biological Sciences, Universiti Sains Malaysia,
11800 Minden, Pulau Pinang, Malaysia.
ABSTRACT
RAPD was used to assess genetic relatedness amongst 59 strains of Fusarium spp. in the section Liseola obtained from rice plants showing bakanae symptoms from different major granary areas in Malaysia and Indonesia, as well as 14 strains of standard testers from U.S.A. RAPD analysis using 10 nucleotides with 60 – 70% G+C contents primers i.e. OPA-02 (5’-tgc-cga-gct-g-3’), OPA-04 (5’-aat-cgg-gct-g-3), OPA-09 (5’-ggg-taa-cgc-c-3’), OPA-17 (5’-gac-cgc-ttg-t-3’), OPT-11 (5’-ttc-ccc-gcg-a-3’), OPT-18 (5’-gat-gcc-aga-c-3’), OPU-06 (5’-acc-ttt-gcg-g-3’), OPU-16 (5’-cgt-cgc-tgg-a-3’), OPV-06 (5’-acg-ccc-agg-t-3’) and OPV-16 (5’-aca-ccc-cac-a-3’) showed variations on DNA profiles between the species, indicating the species were genetically heterogeneous. Dendograms from cluster analysis based on UPGMA showed that the six species of Fusariumwere clustered separately.
ABSTRAK
RAPD telah digunakan untuk menilai hubungan genetik di antara 59 strain Fusarium spp. dalam seksyen Liseola yang diperolehi daripada pokok padi yang menunjukkan symptom penyakit bakanae dari beberapa kawasan penanaman padi di Malaysia dan Indonesia, termasuk 14 strains tester dari U.S.A. Analisis RAPD menggunakan primer yang mempunyai 10 nukleotida dengan 60 – 70% kandungan G+C iaitu OPA-02 (5’-tgc-cga-gct-g-3’), OPA-04 (5’-aat-cgg-gct-g-3), OPA-09 (5’-ggg-taa-cgc-c-3’), OPA-17 (5’-gac-cgc-ttg-t-3’), OPT-11 (5’-ttc-ccc-gcg-a-3’), OPT-18 (5’-gat-gcc-aga-c-3’), OPU-06 (5’-acc-ttt-gcg-g-3’), OPU-16 (5’-cgt-cgc-tgg-a-3’), OPV-06 (5’-acg-ccc-agg-t-3’) dan OPV-16 (5’-aca-ccc-cac-a-3’) menunjukkan variasi ke atas profil DNA di antara spesies, menandakan spesies tersebut secara genetiknya adalah pelbagai. Dendogram dari analisis kluster berasaskan UPGMA mengelaskan enam spesies Fusariumsecara asing.
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