Malays. Appl. Biol. (2005) 34(1): 9-14
PROTEIN PROFILE OF ANABAENA FLOS-AQUAE EXPOSED TO ARSENIC AND HEAVY METALS MANGANESE AND CADMIUM
NIK, M.S.1*, KHAIRIAH, J.2, LIM, J.C.Y.1 and MUSHRIFAH, I.2
'School of Bioscience and Biotechnology,
2School of Environmental Science and Natural Resources,
Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor
ABSTRACT
We report the study on protein profile of Anabaena flos-aquae after exposure to arsenic (As) and two heavy metals manganese (Mn) and cadmium (Cd). This initial study was performed in order to find out if any particular protein is expressed after the heavy metal-resistant A. flos aquae was treated with these metals. The heavy metal concentrations used in the treatment were based on LD50 concentration. A. flos-aquae treated with Mn showed the presence of 6.5, 14 and 20 kDa protein bands and similar to untreated sample. Exposure of A. flos-aquae with Cd at LD5o and 50% above LD5o concentrations suppressed the expression of the 14 and 20 kDa bands. However, additional protein bands of 29, 36 and 55 kDa appeared in the sample treated with Cd 50 % higher than the LD50 concentration. Whereas, A. flos-aquae treated with 50% above LD50 concentration shows the presence of 24 and 55 kDa bands. Treatment of A. flos-aquae with As produced the same protein bands as the untreated sample except that at LD50 concentration, the 20 kDa band disappeared. However, the 14 kDa band is expressed at higher level in sample treated with concentration 50 % lower than LD50 and at LD50 concentrations, before it disappeared again at 50 % above LD50 concentration. These results suggest that the 55 kDa protein is very potential to be used as a biosensor for Mn contamination.
ABSTRAK
Kami melaporkan kajian profil protein pada Anabaena flos-aquae yang diberikan pendedahan arsenik (As) dan dua jenis logam berat iaitu mangan (Mn) dan kadmium (Cd). Kajian ini dijalankan untuk melihat sama ada terdapat protein tertentu yang diekspreskan selepas A. flos-aquae yang rintang terhadap logam diberikan perlakuan dengan logam berat tersebut. Kepekatan logam berat yang digunakan dalam perlakuan ini adalah berasaskan kepekatan pada LD50. A. flos-aquae yang diberikan perlakuan dengan Mn menunjukkan kehadiran jalur protein 6.5, 14 dan 20 kDa yang sama seperti sampel yang tidak diberikan sebarang perlakuan. Pendedahan A. flos-aquae dengan Cd pada kepekatan LD50 dan 50% tinggi dari kepekatan LD50 didapati menekan pengekpresian jalur 14 dan 20 kDa. Walau bagaimanapun, protein tambahan berberat molekul 29, 36 dan 55 kDa di perhatikan nadir pada sampel yang diberikan perlakuan dengan kepekatan Cd 50 % lebih tinggi dari kepekatan LD50. Manakala perlakuan A. flos-aquae pada kepekatan 50% di atas LD50 menunjukkan kehadiran jalur 24 dan 55 kDa. Perlakuan A. flos-aquae dengan As menghasilkan jalur protein yang sama seperti sampel kawalan kecuali pada kepekatan LD50 didapati jalur 20 kDa menghilang Walau bagaimanapun jalur 14 kDa telah diekspreskan pada paras yang lebih tinggi pada sampel yang yang diperlakukan dengan kepekatan LD50dan 50% di bawah kepekatan LD50 perlakuan pada kepekatan 50% lebih tinggi daripada LD50 menyebabkan jalur 14 kDa tersebut menghilang Keputusan ini mencadangkan bahawa protein pada 55 kDa sangat berpotensi untuk digunakan sebagai biosensor untuk pencemaran Mn.
Key words: heavy metals, Anabaena flos-aquae, protein, pollution
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