Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2005) 34(1): 15-19

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Malays. Appl. Biol. (2005) 34(1): 15-19





1School of Biosciences and Biotechnology,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor Darul Ehsan

E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

2Department of Bioprocess Engineering and Natural Resources

Universiti Teknologi Malaysia, 81310 Skudai, Johor Baharu, Malaysia.


The effect of C:N ratios and temperature on the production of lipid and GLA by four oleaginous filamentous fungi (Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 and Geotrichum sp. SK22) was studied. All isolates showed up to two-fold increase in lipid content with the increase in C:N ratio in the medium. However, the increase in lipid content resulted in the decrease of GLA content (% of total fatty acids). For Absidia sp. DR, the GLA content decreased from 18% at C:N 40:1 to 5% when C:N was increased to 133:1, which corresponded inversely to an increase of lipid from 17% to 32% (g/g biomass). This inverse relationship of lipid and GLA content was also observed in the other three fungi. When Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 were grown at different temperatures (15°C, 20°C, 25°C and 30°C), all showed maximum lipid production at 25 to 30°C. Nevertheless, no significant changes in GLA content was observed at different temperatures.


Kesan nisbah C:N dan suhu pertumbuhan terhadap penghasilan lipid dan GLA oleh empat kulat oleaginous (Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 dan Geotrichum sp. SK22) telah dikaji. Kesemua pencilan kulat menunjukkan peningkatan dua kali ganda dalam kandungan lipid dengan peningkatan nisbah C:N dalam medium. Walau bagaimanapun, peningkatan kandungan lipid telah diikuti dengan dengan pengurangan kandungan GLA (%, dari asid lemak jumlah). Dalam Absidia sp DR, kandungan lipid telah berkurangan dari 18% pada C:N 40:1 kepada 5 % (g/g biojisim) apabila C:N ditingkatkan kepada 133:1 berkadar songsang dengan peningkatan kandungan lipid dari 17% kepada 32% (g/g biojisim). Perkaitan songsang di antara kandungan lipid dan GLA ini juga dicerap di dalam tiga pencilan kulat yang lain. Apabila Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 tiga daripada pencilan -pencilan kulat ini dieram pada suhu yang berbeza (15°C, 20°C, 25°C dan 30°C), kesemuanya menunjukkan penghasilan lipid yang maksimum pada 25°C dan 30°C. Walau bagaimanapun, tiada pefbezaan yang jelas dicerap dari segi kandungan GLA diperhatikan pada suhu yang berbeza.

Keywords: Gamma linolenic acid, carbon and nitrogen ratios, temperature.


Botham, P.A. and Ratledge, C. 1979. A biochemical explanation for lipid accumulation in Candida 107 and other oleaginous micro-organisms. Journal of General Microbiology 114: 361-375.

Chancy, A.L. and Marbach, E.P. 1962. Modified reagents for determination of urea and ammonium. Clinical Chemistry 8: 130-132.

Cheng, M.H., Walker, T.H., Hulbert, G.J. and Raman, D.J. 1999. Fungal production of eicosapentaenoic and arachidonic acids from industrial waste streams and crude soybean oil. Bioresource Technology 67: 101-110.

Evans, C.T. and Ratledge, C. 1985. Possible regulatory roles of ATPicitrate lyase, malic enzymes and AMP: deaminase in lipid accumulation by Rhodosporidium toruloides CBS 14. Canadian Journal of Microbiology 31: 1000-1005

Folch, J., Lees, M., Sloane-Stanley, G.H. 1957. A simple method for the isolation of total lipids from animal tissues. Journal of Biological Chemistry 226: 497-506.

Hamid, A.A., Yusoff, W.M.W., Illias, R.M. and Nadarajah, K. 2001. Pengasingan strain baru kulat dari tanah di Malaysia untuk penghasilan asid -g- linolenik (GLA), Jurnal Teknologi 34:1-8.

Horrobin, D.F. 1979. Schizophrenia: Reconciliation of the dopamine, prostaglandin and opoid concepts and the role of pineal. Lancet 529.

Horrobin, D.F. 1992. Clinical applications of n-6 essential fatty acids: atopic eczema and inflammation, diabeticneuropathy and retinopathy, breast pain and viral infection, In: essential fatty acids and eicosanoids, 3rd International Congress. Sinclair, A., Gibson, R.( Eds.). Champaign:IL: American Oil Chemists' Society, pp 367-372

Jantti, J., Seppala, E., Vapaatalo, H., Isomaki, H. 1989. Evening primrose oil and olive oil in treatment of rheumatoid arthritis. Clinical Rheumatology. 8: 238-244

Kendrick, A. and Ratledge, C. 1992. Desaturation of polyunsaturated fatty acids in Mucor circinelloides and the involvement of the novel membrane-bound malic enzyme. European Journal of Biochemistry 209: 667-673

Kennedy, M.J., Reader, S.L and Davies, R.J. 1993. Fatty acid production characteristics of fungi with particular emphasis on gamma-linolenic acid production. Biotechnology and Engineering 42: 625-634.

Khoo, S.K., Munro, C., Battistutta, D. 1990. Evening primrose oil and treatment of premenstrual syndrome. Med. J. Australia 153: 189-192.

Lindberg, A.M. and Molin, G. 1993. Effects of temperature and glucose supply on the production of polyunsaturated fatty acids by the fungus Mortierella alpina CBS 343.66 infermenter cultures. Applied Microbiology and Biotechnology 39: 450-455.

Menendez, J.A., Mar Barbacid, M.D., Montero, S., Sevilla, E., Escrich, E., Solanas, M., Cortes-Funes, H. and Colomer, R. 2001. Effects of gamma-linolenic acid and oleic acid on paclitaxel cytotoxicity in human breast cancer cells. European Journal of Cancer 37: 402-413.

Ratledge, C. 1989. Biotechnology of oils and fats. In: Microbial Lipids. C. Ratledge, S. G. Wilkinson ( Eds.) Vol. 2, London, Academic Press, pp. 567-668. Ratledge, C. 1992. Microbial Lipids: Commercial Realities or Academic Curiosities. In Industrial application of single cell oil. D.J., Kyle, and C. Ratledge (Eds.). Champaign: Illinois, USA. pp. 1-15.

Ratledge, C. 1997. Microbial lipids In : Biotechnology. Rehm, H. J. and Reed, G. (Eds.). VCH, Germany. Pp 159-160. Sajbidor, J, Dobronova, S. and Certik, M. 1990. Arachidonic acid production by Mortierella sp. S-17: influence of C/N ratio. Biotechnology Letters 12: 455-456.

Sattur, A.P. and Karanth, N.G. 1989. Production of microbial lipids: III. Influence of C/N ratio - experimental observations. Biotechnology and Bioengineering 34: 872-874.

Shimizu, S., Shinmen, Y., Kawashima, H., Akimoto, K. and Yamada, H. 1988. Fungal mycelia as a novel source of eicosapentaenoic acid: Activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature. Biochem. Biophys. Res. Commun. 150: 335-341.

Singh, A. and Ward, O.P. 1997. Production of high yields of arachidonic acid in a fed-batch system by Mortierella alpina ATCC 32222. Applied Microbiology and Biotechnology. 48: 1-5.

Wynn, J.P., Hamid, A.A., Li, Y. and Ratledge, C. 2001. Biochemical events leading to the diversion of carbon into storage lipids in oleaginous fungi Mucor circinelloides and Mortierella alpina. Microbiology 147: 2857-2864.



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