Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2005) 34(1): 15-19

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Malays. Appl. Biol. (2005) 34(1): 15-19

THE EFFECT OF CARBON AND NITROGEN RATIOS AND

TEMPERATURE ON LIPID AND G-LINOLENIC ACID (GLA)

YIELD IN FOUR FUNGAL ISOLATES

AIDIL, A.M.1*, ROSLI, M.I.2 and WAN-MOHTAR, W.Y.1

1School of Biosciences and Biotechnology,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor Darul Ehsan

E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

2Department of Bioprocess Engineering and Natural Resources

Universiti Teknologi Malaysia, 81310 Skudai, Johor Baharu, Malaysia.

ABSTRACT

The effect of C:N ratios and temperature on the production of lipid and GLA by four oleaginous filamentous fungi (Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 and Geotrichum sp. SK22) was studied. All isolates showed up to two-fold increase in lipid content with the increase in C:N ratio in the medium. However, the increase in lipid content resulted in the decrease of GLA content (% of total fatty acids). For Absidia sp. DR, the GLA content decreased from 18% at C:N 40:1 to 5% when C:N was increased to 133:1, which corresponded inversely to an increase of lipid from 17% to 32% (g/g biomass). This inverse relationship of lipid and GLA content was also observed in the other three fungi. When Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 were grown at different temperatures (15°C, 20°C, 25°C and 30°C), all showed maximum lipid production at 25 to 30°C. Nevertheless, no significant changes in GLA content was observed at different temperatures.

ABSTRAK

Kesan nisbah C:N dan suhu pertumbuhan terhadap penghasilan lipid dan GLA oleh empat kulat oleaginous (Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 dan Geotrichum sp. SK22) telah dikaji. Kesemua pencilan kulat menunjukkan peningkatan dua kali ganda dalam kandungan lipid dengan peningkatan nisbah C:N dalam medium. Walau bagaimanapun, peningkatan kandungan lipid telah diikuti dengan dengan pengurangan kandungan GLA (%, dari asid lemak jumlah). Dalam Absidia sp DR, kandungan lipid telah berkurangan dari 18% pada C:N 40:1 kepada 5 % (g/g biojisim) apabila C:N ditingkatkan kepada 133:1 berkadar songsang dengan peningkatan kandungan lipid dari 17% kepada 32% (g/g biojisim). Perkaitan songsang di antara kandungan lipid dan GLA ini juga dicerap di dalam tiga pencilan kulat yang lain. Apabila Cunninghamella sp. 2A1, Absidia sp. DR, Bortrytis sp. K3 tiga daripada pencilan -pencilan kulat ini dieram pada suhu yang berbeza (15°C, 20°C, 25°C dan 30°C), kesemuanya menunjukkan penghasilan lipid yang maksimum pada 25°C dan 30°C. Walau bagaimanapun, tiada pefbezaan yang jelas dicerap dari segi kandungan GLA diperhatikan pada suhu yang berbeza.

Keywords: Gamma linolenic acid, carbon and nitrogen ratios, temperature.

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