Malays. Appl. Biol. (June 2004) 33(1): 19-25
COMPARISONS OF ENDOGLUCANASES FROM ANAEROBIC
RUMEN BACTERIA FIBROBACTER SUCCINOGENES AND
RUMINOCOCCUS FLAVEFACIENS
WONG, C.M.V.L.1*, SIEO, C.C.2, ABDULLAH, N.2 and HO, Y.W.3
1Biotechnology Research Institute, Universiti Malaysia Sabah,
Locked Bag 2073, 88999 Kota Kinabalu, Sabah, Malaysia.
E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
2Department of Biochemistry and Microbiology,
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
ABSTRACT
Four strains of Fibrobacter succinogenes (Dl, D3, D5 and D6) and three strains of Ruminococcus flavefadens (MD-1, MD-8 and MD-9) isolated from the rumen of sika deer (Cervus nippon) and lesser mousedeer (Tragulus javanicus), respectively, were found to be highly fibrolytic, degrading and solubilizing Whatman No.l filter paper strips in anaerobic Scott and Dehority medium within 16-48 hrs. Among the seven bacterial strains, F. succinogenes D3 and R. flavefadens MD-1 were the most active, with the ability to solubilize the filter paper strips within 16-24 hrs. These isolates were _:sed for endoglucanase assay. Ruminococcus flavefadens MD-1 produced significantly (P<0.05) higher endoglucanase ictivity than F. succinogenes D3 at all incubation periods. Analysis of endoglucanase using polyacrylamide gel riectrophoresis revealed that both F. succinogenes D3 and R. flavefadens MD-1 possessed three endoglucanase isozymes : -t the endoglucanase bands for each bacterial strain were different, indicating that the endoglucanases produced by the 7*0 strains are different.
ABSTRAK
Ernpat strain Fibrobacter succinogenes (Dl, D3, D5 dan D6) yang telah dipencil dari pelanduk sika (Cervus nippon) din tiga strain Ruminococcus flavefadens (MD-1, MD-8 dan MD-9) yang telah dipencil dari kancil (Tragulus javanicus) ~empunyai aktiviti fibrolitik yang tinggi dan berupaya menghadamkan cebisan kertas turas Whatman No. 1 dalam media Scott dan Dehority dalam jangkamasa 16-48 jam. Di antara tujuh strain bakteria, F. succinogenes D3 dan R. flavefadens MD-l adalah dua strain bakteria yang paling aktif, dan yang dapat menguraikan cebisan kertas turas dalam tempoh 16-24 jam. Kedua-dua strain telah digunakan untuk assai aktiviti endoglukanase. Ruminococcus flavefadens MD-1 •cnghasilkan aktiviti endoglukanase yang nyata (P<0.05) lebih tinggi berbanding dengan F. succinogenes D3 pada setiap peringkat masa pengeraman. Analisa endoglukanase dengan menggunakan kaedah elektroforesis gel poliakrilamida (PAGE) ;:.unjukkan bahawa kedua-dua F. succinogenes D3 dan R. flavefadens MD-1 menghasilkan tiga isozim endoglukanase _-; jalur endoglukanase bagi kedua-dua strain bakteria berada pada kedudukan yang berbeza pada gel poliakrilamida aenunjukkan bahawa endoglukanase-endoglukanase yang dihasilkan oleh kedua-dua strain ini adalah berbeza.
Keywords: Rumen bacteria, Fibrobacter succinogenes, Ruminococcus flavefadens, endoglucanase.
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