Malays. Appl. Biol. (June 2004) 33(1): 53-60
SOLID STATE FERMENTATION FOR LIPASE PRODUCTION BY
A LOCALLY ISOLATED ASPERGILLUS TERREUS USM E5
USING RUBBERWOOD SAWDUST AS SUBSTRATE
HOW, S.P., OOI, A.N. and IBRAHIM, C.O.*
Fermentation and Enzyme Technology Laboratory,
School of Biological Sciences, Universiti Sains Malaysia,
Minden, 11800 Penang, Malaysia.
ABSTRACT
An indigenous fungal isolate which was identified as Aspergillus terreus USM E5 was selected as a potential producer of lipase using solid state fermentation (SSF) with rubberwood saw dusts as the substrate. Under the optimized cultivation conditions consisting of temperature 30°C, average substrate particle size 0.5 mm, water content 100% (v/w), inoculum size 1 x 105 spores/ml and 10% (w/w) of olive oil as inducer, a maximum production of lipase of 18.3 U/g substrate and growth of 1.03 x 10"3 mg glucosamine/g substrate was obtained after 4 days of fermentation. The lipase productivity after optimization increased 3-folds with 4.58 U/g substrate/day compared to before optimization which was only 1.50 U/g substrate/day. The lipase production by the fungus is parallel to the growth profile of the fungus.
ABSTRAK
Satu pencilan kulat tempatan yang dicamkan sebagai Aspergillus terreus USM E5 telah dipilih sebagai penghasil lipase yang berpotensi menggunakan pemfermentasian keadaan pepejal (SSF) dengan habuk kayu getah sebagai substrat. Dibawah keadaan optimum yang terdiri daripada suhu 30°C, saiz substrat purata 0.5 mm, kandungan air 100% (i/b), saiz inokulum 1 x 105 spora/ml dan minyak zaitun 10% (b/b) sebagai bahan aruh, penghasilan maksimum sebanyak 18.3 U/g substrat dan pertumbuhan 1.03 x 10"3 mg glukosamina/g substrat diperoleh selepas 4 hari pemfermentasian. Daya pengeluaran selepas pengoptimuman meningkat 3 kali ganda sebanyak 4.58 U/g substrat/hari berbanding sebelum pengoptimuman yang hanya sebanyak 1.50 U/g substrat/hari. Penghasilan lipase oleh kulat ini adalah selari dengan profil pertumbuhan kulat.
Key words: solid state fermentation, Aspergillus terreus, lipase production, rubberwood saw dusts
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