Malays. Appl. Biol. (June 2003) 32(1): 73-79
ESTIMATION OF GENETIC RELATEDNESS AMONG ACACIA MANGIUM X ACACIA AURICULIFORMIS HYBRIDS
*WICKNESWARI, R., KOH, S.C., THI, B.K., LOW, C.Y. and TAN, E.
School of Environmental and Natural Resource Sciences
Faculty of Science and Technology
Universiti Kebangsaan Malaysia
43600 UKM, Bangi, Selangor, Malaysia
ABSTRACT
Directed Amplification of Minisatellite DNA regions (DAMD) using M13 universal primer generated 12 unique and 4 identical DNA profiles for 22 Acacia hybrids. Eighteen different ISTR primer combinations generated unique DNA fingerprints for all the 20 Acacia hybrids with 10 fold lower possibility of obtaining two identical ISTR fingerprints (0.00659) than minisatellite fingerprints (0.066). Identity genetic value (J) obtained was 0.599 for F] hybrids and 0.731 for F2 hybrids. ISTR was more suitable than DAMD for estimating genetic relatedness and DNA fingerprinting analysis in Acacia hybrids.
ABSTRAK
Amplifikasi Terarah DNA Minisatelit dengan menggunakan pencetus universal M13 menghasilkan 12 profil DNA yang unik dan 4 profil yang seiras untuk 22 hibrid Acacia. Lapan belas kombinasi pencetus ISTR yang berlainan menghasilkan pengecapjarian DNA yang unik untuk kesemua 20 hibrid Acacia. Kebarangkalian untuk memperolehi 2 pengecapjarian ISTR yang seiras adalah 10 kali lebih rendah (0.00659) berbanding pengecapjarian minisatelit (0.066). Nilai identiti genetik (J) yang diperolehi untuk hibrid F[ ialah 0.599 manakala untuk hibrid F2 ialah 0.731. Kaedah ISTR adalah lebih sesuai berbanding kaedah DAMD bagi analisis hubungan genetik dan pengecapjarian DNA untuk hibrid Acacia.
Key words: minisatellite, ISTR, DNA fingerprinting, genetic relatedness, Acacia hybrids.
REFERENCES
Bowen, M.R. 1981. Acacia mangium. A note on seed collection, handling and storage techniques including some experimental data and information on A. auriculiformis and the probable A. mangium x A. auriculiformis hybrid. Occassional Technical and Scientific Notes, Seed Series Number 3. FAO/UNDP-MAL/78/009, Forest Research Centre, Sandakan, Sabah.
Butcher, P.A. and Moran, G.F. 2000. Genetic linkage mapping in Acacia mangium. 2. Development of an intergrated map from two outbred pedigrees using RFLP and microsatellite loci. Theoretical and Applied Genetics 101: 594-605.
Butcher, P.A., Moran, G.F. and Bell, R. 2000. Genetic linkage mapping in Acacia mangium 1. Evaluation of restriction endonucleases, inheritance of RFLP loci and their conservation across species. Theoretical and Applied Genetics 100: 576-583.
Chong, D.K.X., Yen, F.C., Aravanopoulos, F.A. and Zsuffa, L. 1995. DNA fingerprinting willows (Salix L.) using polymerase chain reaction with the M13 universal primer . Scandinavian Journal of Forest Research 10: 27-31.
Doyle, J.J. and Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
Rohde, W. 1996. Inverse sequence-tagged repeat (ISTR) analysis, a novel and universal PCR-based technique for genome analysis in the plant and animal kingdom. Journal of Genetics and Breeding 50: 249-261.
Rohlf, F.J. 1990. Numerical Taxonomy and Multivariate Analysis NTSYS-pc. USA: State University of New York.
Rufelds, C.W. 1988. Acacia mangium, A. auriculiformis and hybrid A. auriculiformis
seedling morphology study. FRC Publication No. 41. pp. 83.
Rufelds, C.W. and Lapongan, J. 1986. The occurrence of hybrid Acacia auriculiformis A. Cunn. ex Benth. in Sabah. Proceedings of the Ninth Malaysian Forestry Conference, Kuching, Sarawak, August 1986.
Wickneswari, R. 1989. Use of isozyme analysis in a proposed Acacia mangium x Acacia auriculiformis hybrid seed production orchard. Journal of Tropical Forest Science 2(2): 157-164.
Wickneswari, R. and Lee, C.T. 1993. Clonal identification of Acacia mangium x Acacia auriculiformis hybrids using RAPD markers. Malavsian Forester 56(1): 36-42
