Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2009) 38(2): 1–4



¹Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
²Department of Physic, Faculty of Science, University of Isfahan, Isfahan, Iran
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Gamma rays as an electromagnetic spectrum can induce different types of DNA damages such as single and double strand breaks. The comet assay is a useful tool for detection of DNA break. In this study potato callus cultivar White Desiree were irradiated with doses of 60 and 80 Gy of gamma rays. Immediately after irradiation cell viability and DNA breaks were analyzed. Doses of 60 and 80 Gy of radiations showed single and double strand breaks based on extension of nuclei (comet tail length). Viability of callus cell was 95-100%. After 24h DNA damages were repaired and length of comet was similar to un-radiated calli.

Key words: callus, cell viability, comet assay, DNA break, potato


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