Malays. Appl. Biol. (2009) 38(2): 1–4
DETECTION OF DOUBLE AND SINGLE STRAND DNA BREAKS IN GAMMA IRRADIATED POTATO CALLUS USING COMET ASSAY
BEHROKH SHOJAIE¹, ALI AKBAR EHSANPOUR¹* and MOHAMMAD REZA ABDI²
¹Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
²Department of Physic, Faculty of Science, University of Isfahan, Isfahan, Iran
*E-mail:
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
ABSTRACT
Gamma rays as an electromagnetic spectrum can induce different types of DNA damages such as single and double strand breaks. The comet assay is a useful tool for detection of DNA break. In this study potato callus cultivar White Desiree were irradiated with doses of 60 and 80 Gy of gamma rays. Immediately after irradiation cell viability and DNA breaks were analyzed. Doses of 60 and 80 Gy of radiations showed single and double strand breaks based on extension of nuclei (comet tail length). Viability of callus cell was 95-100%. After 24h DNA damages were repaired and length of comet was similar to un-radiated calli.
Key words: callus, cell viability, comet assay, DNA break, potato
REFERENCE
Baek, M.-H., Kim, J.-H., Chung, B.Y., Kim, S.-J. & Lee, I.S. 2005. Alleviation of salt stress by low dose g irradiation in rice. Biologia Plantarum, 49(2):273–276.
Buiatti, M. & Ragazzini, R. 1965. Gamma-ray induced changes in the carnation, Dianthus caryophyllusL. Radiation Botany, 5(2): 99–105.
Chung, B.Y., Lee, Y.-B., Baek, M.-H., Kim, J.-H., Wi, S.G. & Kim, J.-S. 2006. Effects of low-dose gamma-irradiation on production of shikonin derivatives in callus cultures of Lithospermum erythrorhizonS. Radiation Physics and Chemistry, 75:1018–1023.
Faure, V., Saparbaev, M., Dumy, P. & Constant, JF. 2005. Action of multiple base excision repair enzymes on the 2-deoxyribonolactone. Biochemical and Biophysical Research Communications, 328:1188–1195.
Gichner, T. & Plewa, M.J. 1998. Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutation Research, 401:143–152.
Gichner, T., Patkova, Z., Szakova, J. & Demnerova, K. 2006. Toxicity and DNA damage in tobacco and potato plants growing on soil polluted with heavy metals. Ecotoxicology and Environmental Safety, 65:420–426.
Hayashi, H., Narumi, I., Wada, S., Kikuchi, M., Furuta, M., Uehara, K. & Watanabe, H. 2004. Light dependency of resistance to ionizing radiation in Euglena gracilis. Journal of Plant Physiology, 161:1101–1106.
Hefner, E., Huefner, N. & Britt, A.B. 2003. Tissue-specific regulation of cell-cycle responses to DNA damage in Arabidopsisseedlings. DNA Repaire, 5:102–110.
Hefner, E., Preuss, S.B. & Britt, A.B. 2006. Arabidopsismutants sensitive to gamma radiation include the homologue of the human repair gene ERCC1. Journal of Experimental Botany, 54:669–680.
Heslop-Harrison, J.Y. & Shivanna. K.R. 1984. The evaluation of pollen quality, and a further appraisal of the fluorochromatic (FCR) test procedure. Theoretical and Applied Genetics, 67:367–375.
Koppen, G. & Verschaeve, L. 1996. The alkaline comet test on plant cell: A new genotoxicity test for DNA strand breaks in Vicia faba root cell. Mutation Research, 360:193–200.
Kovacs, E. & Keresztes, A. 2002. Effect of gamma and UV-B/C radiation on plant cells. Micron, 33:199-210.
Maity, J.P., Mishra, D., Chakraborty, A., Saha, A., Santra, S.C. & Chanda, S. 2005. Modulation of some quantitative and qualitative characteristics in rice (Oryza sativaL.) and mung (Phaseolus mungoL.) by ionizing radiation. Radiation Physics and Chemistry, 74:391–394.
Misra, P., Datta, S.K. & Chakrabarty, D. 2003. Mutation in flower colour and shape of Chrysanthemum morifoliuminduced by ? radiation. Biologia Plantarum, 47(1):153–156.
Miyazaki, T., Morikawa, A., Kumagai, J., Ikehata, M., Koana, T. & Kikuchi, S. 2002. Long-lived radicals produced by ã-irradiation or vital activity in plants, animals, cells, and protein solution: their observation and inhomogeneous decay dynamics. Radiation Physics and Chemistry, 65: 151–157.
Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15:473–497.
Nagata, T., Todoriki, S., Hayashi, T., Shibata, Y., Mori, M., Kanegae, H. & Kikuchi, S. 1999 ? Radiation induces leaf trichome formation in Arabidopsis. Plant Physiology, 120:113–119.
Naito, K., Kusaba, M., Shikazono, N., Takano, T., Tanaka, A., Tanisaka, T. & Nishimura, M. 2005. Transmissible and nontransmissible mutations induced by irradiating Arabidopsis thaliana pollen with ?-rays and carbon ions. Genetics, 169:881–889.
Navarrete, M.H., Carrera, P., De Miguel, M. & De la Torre, C. 1997. A fast comet assay variant for solid tissue cells. The assessment of DNA damage in higher plants. Mutation Research, 389:271–277
Ostling, O. & Johanson, K.J. 1984. Microelectrophoretic study of radiation-induced DNA damage in individual mammalian cell. Biochemical and Biophysical Research Communications, 123:291–298.
Pincheira, J., Lopez-Saez, J.F., Carrera, P., Navarrete, M.H. & De la Torre, C. 2003. Effect of caffeine on in vivoprocessing of alkylated bases in proliferating plant cells. Cell Biology International, 27:837–843.
Ptacek, O., Stavreva, D.A., Kim, J.K. & Gichner, T. 2001. Induction and repair of DNA damage as measured by the Comet assay and the yield of somatic mutations in gamma-irradiated tobacco seedlings. Mutation Research, 491: 17–23.
Singh, N.P., MacCoy, M.T., Tice, R.R. & Schneider, E.L. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental of Cell Research, 175:184–191.
Veleminky, J. & Van’t Hof, J. 1984. Repair of X-ray induced single-strand breaks in root tips of Tradescanita clones 02 and 4430. Mutation Research, 131:143–146.
Villavicencio, A.L.C.H., Arau’jo, M.M., Baldasso, J.G., Aquino, S., Konietzny, U. & Greiner, R. 2004. Irradiation influence on the detection of genetic-modified soybeans. Radiation Physics and Chemistry, 71:489–492.
