Malays. Appl. Biol. (2009) 38(2): 49–54
APPLICATION OF SILVER THIOSULFATE (STS) ON SILVER ACCUMULATION AND PROTEIN PATTERN OF POTATO (Solanum tuberosum L.) UNDER IN VITRO CULTURE
ROSTAMI, F. and EHSANPOUR, A.A.*
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
*E-mail:
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
ABSTRACT
The effect of silver thiosulfate (STS) on silver accumulation and protein pattern changes was investigated in potato (Solanum tuberosum L.) cultivar White Desiree. In vitro grown plants were subjected to MS medium containing concentrations of 0, 50, 100, 150 and 200 ?M silver thiosulfate (STS) for six weeks. Application of Different concentrations of STS had not significant effect on the total soluble proteins while, protein pattern of stem-leaf and root tissues showed some remarkable changes using poly acrylamide gel electrophoresis (SDS-PAGE) and consequence analysis of bands by ImageJ program. Relative expression of four proteins in stem-leas and five proteins in root were changed in response to STS treatment. Silver accumulation was detected in root tissue only. Results showed that using STS as ethylene inhibitor improve growth and development by alternation of some proteins involve in growth process.
Key words: ethylene, potato, protein pattern, silver thiosulfate (STS), SDS-PAGE, Solanum tuberosum L., total soluble protein, tissue culture
REFERRENCES
Abeles, F.B., Dunn, L.J., Morgens, P., Callahan, A., Dinterman, R.E. and Schmidt, J. 1988. Induction of 33-KD and 60-KD peroxidases during ethylene-induced senescence of cucumber cotyledons. Plant Physiology, 87: 609-615.
Amini, F., Ehsanpour, A.A., Hoang, Q.T. and Shin, J.Sh. 2007. Protein pattern changes in tomato under in vitro salt stress. Russian Journal of Plant Physiology, 54(4): 464-471.
Beyer, E.M. 1979. Effect of Silver Ion, Carbon Dioxide, and Oxygen on Ethylene Action and Metabolism. Plant Physiology, 63: 169-173.
Bradford, M. 1976. A rapid and sensitive method for the quantitayion of microgram quantities of protein utilizing the principle of protein-dye binding. Annual of Biochemistry, 72: 248-254.
Davies, KM., Hobson, G.E. and Grierson, D. 1988. Silver ion inhibit the ethylene-stimulated production of ripening-related mRNA in tomato. Plant, Cell and Environment, 11(8): 729-738.
Dose, K. 1980. Biochemie. Springer Berlin, Heidelberg, New York.
Ehsanpour, A.A. and Jones, M.G.K. 2001. Plant regeneration from mesophyll protoplasts of potato (Solanum tuberosum L.) cultivar Delaware using silver thiosulfate (STS). Journal of Sciences Islamic Republic of Iran, 12(2): 103- 110.
Ewais, E.A. 1997. Effect of cadmium, nickel and lead on growth, chlorophyll content and proteins of weeds. Biologia Plantarum, 39(3): 403-410.
Hames, B.D. 1990. One-dimensional polyacrylamidegel electrophoresis. In: Gel electrophoresis ofproteins. (eds. Hames, B.D. and Rickwood, D.)2nd ED. Oxford University Press, New York.pp. 382.
Hieng, B., Ugrinovich, K., Sustar-Vozlich, J. andKidric, M. 2004. Different classes of proteasesare involved in the response to drought ofPhaseolus vulgaris cultivars differing insensitivity. Journal of Plant Physiology, 161:519-530.
Leita, L., De Nobili, M., Cesco, S. and Mondini, C.1996. Analysis of intercellular cadmium formsin roots and leaves of bush bean. Journal ofPlant Nutrition, 19: 527-533.
Murashige, T. and Skoog, F. 1962. A revisedmedium for rapid growth and bioassays withtobacco tissue cultures. Physiologia Plantarum,15: 473-497.
Nishizono, G., Kubota, K., Suzuki, S. and Ishii, F.1989. Accumulation of heavy metals in cellwalls of Polygonum cuspidatum roots frommetalliferous habitate. Plant Cell Physiology,30: 595-598.
Olson, B.J.S.C. and Markwell, J. 2007. Currentprotocols in protein science. Detection andAssay Method, 48: 3.4.1-3.4.29.
Orczyk, W., Przetakiewicz, J. and Nadolska-Orczyk,A. 2003. Somatic hybrids of Solanum tuberosum– application to genetics and breeding. PlantCell, Tissue and Organ Culture, 74: 1-13.
Perl, A., Aviv, D. and Galun, E. 1988. Ethylene andin vitro culture of potato: suppression ofethylene generation vastly improves protoplastyield, platingefficiency and transient expressionof an alien gene. Plant Cell Reports, 7(6): 403-406.
Pettersson, O. 1976. Heavy-metal ion uptake byplants from nutrient solutions with metal ion,plant species and growth period variations. Plantand Soil, 45(2): 445-459.
Reeves, R.D., Baker, A.J.M., Barhidi, A. andBerazain, R. 1999. Nickel hyperaccumulation inthe serpentin flora of Cuba. Annals of Botany,83: 29-38.
Rivetta, A., Negrini, N. and Cocucci, M. 1997.Involvement of Ca2+-calmodolin in Cd2+ toxicityduring the early phases of radish (Raphanussativus L.) seed germination. Plant Cell andEnvironment, 20: 600-608
Sarkar, D., Kaushik, S.K. and Naik, P.S. 1999.Minimal growth conservation of potatomicroplants: silver thiosulfate reduces ethylene-induced growth abnormalities during prolongedstorage in vitro. Plant Cell Reports, 18: 897-903.
Sarkar, D., Sud, K.C., Chakrabarti, S.K. and Naik, P.S. 2002. Growing of potato microplants in the presence of alginate-silver thiosulfate capsules reduces ethylene-induced culture abnormalities during minimal growth conservation. In vitro. Plant Cell, Tissue and Organ Culture, 68: 79-89.
Toppi, L.S.D. and Gabbrielli, R. 1999. Response to cadmium in higher plants. Environmental and Experimental Botany, 41: 105-130.
Veen, H. 1983. Silver thiosulfate: An experimental tool in plant science. Scencei. Horticulture, 20: 211-224.
Vierstra, R.D. 1993. Protein degradation in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 44: 385-410.
