Malays. Appl. Biol. (December 2000) 29(1&2): 75-78
CHROMIUM TOXICITY ON ANABAENA FLOS-AQUAE
KHAIRIAH, J., MUSHRIFAH, I. and TAN, J.
Program Sains Sekitaran, Pusat Pengajian Sains Sekitaran dan Sumber Alam Fakulti Sains dan Teknologi UKM, 43600 Bangi, Selangor, Malaysia
ABSTRACT
Chromium was introduced to batch cultures of Anabaena flos-aquae at various concentration of 0.5 mg/1, 1.5 mg/1, 2.0 mg/1 and 4.0 mg/1. Relative to the control there is an increase in growth of A. flos-aquae that were exposed to 0.5 mg/1, 1.0 mg/1, 2.0 mg/1. Growth of cultures were inhibited at the higher Cr concentration. The 96hr LD50 oi A. flos-aquae exposed to Cr was 2.44 mg/1. The amount of Cr absorbed by A. flos-aquae at various Cr concentrations were 66.2%, 31.1%, 17.9%, 14.0% and 9.3% dry weight respectively.
ABSTRAK
Logam kromium telah didedahkan kepada kultur kelompok Anabaena flos-aquae pada kepekatan yang berbeza meliputi 0.5 mg/1, 1.0 mg/1, 1.5 mg/1, 2.0 mg/1, 2.5 mg/1 dan 4.0 mg/1. Terdapat peningkatan pertumbuhan A. flos-aquae secara relatif berbanding dengan kawalan pada kepekatan 0.5 mg/1, 1.0 mg/1, 1.5 mg/1 dan 2.0 mg/1. Perencatan pada pertumbuhan A. flos-aquae berlaku pada kepekatan Cr yang lebih tinggi. Nilai dos maut 96jLD50 Cr untuk A. flos-aquae ialah 2.44 mg/1. Kepekatan Cr yang diserap oleh A. flos-aquae pada kepekatan yang diberikan adalah 66.2%, 31.1% 17.9%, 14.0% dan 9.3% perberat kering.
Key words: Chromium, Anabaena flos-aquae, freshwater, toxicity, mortality.
REFERRENCES
Bishop, N.I. 1964. Site of action of copper in photosynthesis. Nature. 204: 401-411
Bonner, J. and Varner, J.E. 1965. Plant biochemistry. Academic Press. New York.
Bowen, H.J.M. 1966. Trace elements in biochemistry. Academic Press. London.
Corder, S.L. and Reeves, M. 1994. Bioabsorption of nickel in complex aqueous waste streams by cyanobacteria. App. Biotechnol. 45-46: 847-859.
Forstner, U. and Wittmann, G.T.W. 1983. Metal pollution in the aquatic environment. Springler-Verlag. Berlin.
Garnham, G.W. and Green, M. 1995. Chromate (VI) uptake by and interactions with cyanobacteria. J.Ind. Michrobiol. 14: 3-4:247-251.
Hochster, R.M. and Quastel, J.M. 1963. Metabolic inhibitors. Vol 11. Academic Press. New York.
Merian, E. 1991. Metals in the environment. VCH Verlagsgesellschaft. Weinheim.
Mushrifah, I. And Peterson, P.J. 1990. Toxicity of cadmium and tin to chlorophyll a and protein content of Anabaena flos-aquae. Microbios Letters. 45: 151-160.
Mushrifah, I. And Peterson, P.J. 1991. Uptake and accumulation of cadmium and tin to the soluble fractions of Anabaena flos-aquae. Biomedical letters. 46: 189-198.
Perkin Elmer. 1989. Atomic absorption spectrophotometer model 1100B, Operators manual. A2-2.
Reed, L.H. and Muench, H. 1938. A sample method for estimating 50% ends points. Am J. Hyg. 27: 493.
Roger, P.A. and S.A. Kulasooriya. 1980. The International Rice Research Institute, Los Banos, Laguna, Philippines.
Shen-Lin and Lin, Wu. 1994. Effects of Cu Concentration minimal uptake and Cu accumulation in protein of copper tolerance and non tolerance Lotus purshianus. Ecotoxicology & Environ Safety. 29: 214-228.
Verma, N., Batta, S. and Rehal, R. 1995. Studies on some cyanobacteria for the selection of bioindicators and bioscavangers of chromium metal ions for industrial waste waters. Int. J. Environ. Stud. 47: 3-4:211-215.
