Malays. Appl. Biol. (December 2000) 29(1&2): 127-132
MOLECULAR PHYLOGENY OF MELIACEAE BASED ON CHLOROPLAST trnL-trnf NUCLEOTIDE SEQUENCES
DON, B.L., CHOONG, C.Y., MAHANI, M.C. and MAT-SALLEH, K.
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia
ABSTRACT
Phylogenetic relationships between genera from two main subfamilies of the family Meliaceae were inferred from chloroplast trnL-trnF intergenic spacer sequence data. The phylogeny obtained was largely in accordance with the morphological classification. The results indicated strong support for the monophyly of family Meliaceae. Two mair clades corresponding to the two subfamilies Melioideae and Swietenioideae were obtained from the phylogenetic analysis. Within the Melioideae clade, members from tribes Aglaieae, Guareeae and Trichilieae including the economically important species Lansium domesticum formed a subclade and members from tribes Melieae and Sandoriceae formed another subclade that was sister-clade to the former subclade. The phylogenetic relationships among the tribes Aglaieae, Guareeae and Trichilieae remain unresolved. Tribes Cedreleae and Swietenieae formed the Swietenioideae clade. However, tribe Swietenieae appeared to be paraphyletic as Chukrasia tabularis, a member of the tribe, located at the base of the Swietenioideae clade.
ABSTRAK
Hubungan filogeni antara genus daripada dua subfamili utama famili Meliaceae ditentukan berasaskan data jujukan penjarak trnL-trnF daripada genom kloroplas. Filogeni yang diperolehi memperlihatkan pengkelasan berlandaskan morfologi. Hasil keputusan menunjukkan sokongan yang teguh terhadap pembentukan famili Meliaceae yang monofilik. Dua klad utama yang mewakili subfamili Melioideae dan subfamili Swietenioideae terjana daripada analisis filogenetik. Dalam klad Melioideae, anggota-anggota daripada tribus Aglaieae, Guareeae dan Trichilieae yang merangkumi Lansium domesticum membentuk satu subklad manakala anggota-anggota daripada tribus Melieae dan Sandoriceae membentuk satu lagi subklad yang merupakan klad beradik kepada subklad pertama. Hubungan filogenetik di antara tribus Aglaieae, Guareeae dan Trichilieae tidak dapat ditentukan. Tribus Cedreleae dan Swietenieae membentuk klad Swietenioideae. Namun, tribus Switenieae berkeadaan parafiletik kerana salah satu anggota daripada tribus ini (Chukrasia tabularis) ditempatkan pada dasar klad Swietenioideae.
Key words: Phylogenetic, Meliaceae, chloroplast DNA, trnL-trnF intergenic spacer.
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