Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

29-1&2-17

E-mail Print PDF
Malays. Appl. Biol. (December 2000) 29(1&2): 127-132

MOLECULAR PHYLOGENY OF MELIACEAE BASED ON CHLOROPLAST trnL-trnf NUCLEOTIDE SEQUENCES

DON, B.L., CHOONG, C.Y., MAHANI, M.C. and MAT-SALLEH, K.

School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia

ABSTRACT

Phylogenetic relationships between genera from two main subfamilies of the family Meliaceae were inferred from chloroplast trnL-trnF intergenic spacer sequence data. The phylogeny obtained was largely in accordance with the morphological classification. The results indicated strong support for the monophyly of family Meliaceae. Two mair clades corresponding to the two subfamilies Melioideae and Swietenioideae were obtained from the phylogenetic analysis. Within the Melioideae clade, members from tribes Aglaieae, Guareeae and Trichilieae including the economically important species Lansium domesticum formed a subclade and members from tribes Melieae and Sandoriceae formed another subclade that was sister-clade to the former subclade. The phylogenetic relationships among the tribes Aglaieae, Guareeae and Trichilieae remain unresolved. Tribes Cedreleae and Swietenieae formed the Swietenioideae clade. However, tribe Swietenieae appeared to be paraphyletic as Chukrasia tabularis, a member of the tribe, located at the base of the Swietenioideae clade.


ABSTRAK

Hubungan filogeni antara genus daripada dua subfamili utama famili Meliaceae ditentukan berasaskan data jujukan penjarak trnL-trnF daripada genom kloroplas. Filogeni yang diperolehi memperlihatkan pengkelasan berlandaskan morfologi. Hasil keputusan menunjukkan sokongan yang teguh terhadap pembentukan famili Meliaceae yang monofilik. Dua klad utama yang mewakili subfamili Melioideae dan subfamili Swietenioideae terjana daripada analisis filogenetik. Dalam klad Melioideae, anggota-anggota daripada tribus Aglaieae, Guareeae dan Trichilieae yang merangkumi Lansium domesticum membentuk satu subklad manakala anggota-anggota daripada tribus Melieae dan Sandoriceae membentuk satu lagi subklad yang merupakan klad beradik kepada subklad pertama. Hubungan filogenetik di antara tribus Aglaieae, Guareeae dan Trichilieae tidak dapat ditentukan. Tribus Cedreleae dan Swietenieae membentuk klad Swietenioideae. Namun, tribus Switenieae berkeadaan parafiletik kerana salah satu anggota daripada tribus ini (Chukrasia tabularis) ditempatkan pada dasar klad Swietenioideae.

Key words: Phylogenetic, Meliaceae, chloroplast DNA, trnL-trnF intergenic spacer.

REFERRENCES

Bohle, U. R., Hilger, H. H., Cerff, R. and Martin, W. F. 1994. Non-coding chloroplast DNA for plant molecular systematics at the infrageneric level. In: Molecular approaches to ecology ana evolution. R. DeSalle, G. P. Wagner, B. Schierwater and B. Streit (eds.). Birkhauser Verlag, Basel, pp. 391-403.

Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.

Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using bootstrap. Evolution, 39: 783-791.

Fuji, N., Ueda, K., Watano, Y. and Shimizu, T. 1999. Further analysis of intraspecific sequence variation of chloroplast DNA in Primula cuneifolia Ledeb. (Primulaceae): implications for biogeography of the Japanese Alpine Flora. Journal of Plant Research, 112: 87-95.

Gielly, L. and Taberlet, P. 1994. The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences. Molecular Biology and Evolution, 11: 769-777.

Kim, S.-C., Crawford, D. J., Jansen, R. K. and Santos-Guerra, A. 1999. The use of a non-coding region of chloroplast DNA in phylogenetic studies of the subtribe Sonnchinae (Asteraceae: Lactuceae). Plant Systematics and Evolution, 215: 85-99.

Mabberley, D. J. and Pannell, C. M. 1989. Meliaceae. In: Tree flora of Malaya Vol.4. F. S. P. Ng (ed.). Forest Research Institute Malaysia. Kuala Lunnur. dd. 199-260.

Mabberley, D. J., Pannell, C. M. and Sing, A. M. 1995. Meliaceae. Flora Malesiana ser. I, 12: 1-407.

Miller, N. G. 1990. The genera of Meliaceae in the Southeastern United States. Journal of Arnold Arboretum, 71: 453-486.

Pennington, T. D. and Styles, B. T. 1975. A generic monograph of the Meliaceae. Blumea, 22: 419-540.

Song, B.K., Clyde, M.M., Wickneswari, R. and Normah, M.N. 2000. Genetic relatedness among Lansium domesticum accessions using RAPD markers. Annals of Botany, 86:299-307.

Swofford, D. L. 1993. PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1. The Illinois Natural History Survey, Champaign.

Taberlet, P., Gielly, L., Pautou, G. and Bouvet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular and Biology, 17: 1105-1109.

Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The CLUSTAL X windows: flexible strategies for multiple sequencing alignment aided by quality analysis tools. Nucleic Acid Research, 25: 4876-4882.
 

Latest MABJ Issue

Vol 50(1) June 2021

 

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Web of Science Master Journal List, Elsevier, Mycite, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to Scopus CiteScore 2020, MABJ ranks 165 out of 203 Malaysian journals in terms of yearly impact factor.


Scopus Citescore