Malaysian Applied Biology Journal

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Malays. App. Biol. (1997) 26(1): 1-5



1 Department of Microbiology 2 Department of Biochemistry Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia


Batch culture studies of Aspergillus terreus SUK-1 were conducted to evaluate the effect of sodium chloride (NaCl) on the production of cellulase. Results showed that the presence of NaCl (0.05%, 0.10% and 0.50% w/v) in the medium enhancec the production of cellulase. The maximum productivity of CMCase obtained in experiments with 0.05%, 0.10% and 0.50% (w/v) NaCl concentrations were 10.83 x 10"3 lU/ml/hr, 8.33 x 10-1 lU/ml/hr and 7.50 x 10"3 lU/ml/hr respectively. Ir comparison, the maximum CMCase activity in experiments without NaCl was 4.17 x 10"1 lU/ml/hr. The concentration ol reducing sugar was found to increase in the experiments using NaCl, compared to experiments without NaCl. The maximurr reducing sugar obtained in experiments with 0.05%, 0.10% and 0.50% w/v NaCl were 14.40 x 10"' umol/ml 17.80 x 10"' umol/ml and 21.10 x 10"' umol/mi respectively, whereas the maximum reducing sugar in experiment without NaCl was only 5.0 x 10"' umol/ml. Visual observation of the sample at NaCl concentration of 1.00% (w/v) in the medium showec the highest intensity of colour but the highest CMCase activity was low.


Kajian berdasarkan kultur kelompok telah digunakan untuk mengkaji kesan natrium klorida (NaCl) ke atas penghasilar selulase oleh Aspergillus terreus SUK-1. Hasil kajian menunjukkan kehadiran NaCl (0.05%, 0.10% dan 0.50% w/v) dapal meningkatkan produktiviti selulase berbanding dengan eksperimen tanpa NaCl. Produktiviti CMCase bagi eksperimen-eksperimen yang menggunakan 0.05%, 0.10% dan 0.50% (w/v) NaCl masing-masing adalah 10.83 x 10"3 lU/ml/jam 8.33 x 10'3 lU/ml/jam dan 7.50 x 10"3 lU/ml/jam. Sementara nilai maksimum aktiviti CMCase bagi eksperimen tanpa NaCl ialah 4.17 x 10"3 lU/ml/jam. Kandungan gula penurun meningkat dalam eksperimen-eksperimen yang menggunakan NaCl berbanding dengan eksperimen tanpa NaCl. Nilai maksimum gula penurun dalam eksperimen yang dijalankan dengar 0.05%, 0.10% dan 0.50% (w/v) NaCl masing-masing ialah 14.40 x 10"' umol/ml, 17.80 x 10"' umol/ml dan 21.10 x 10"' umol/ml sementara nilai maksimum gula penurun bagi eksperimen tanpa NaCl ialah 5.0 x 10"' umol/ml. Keamatan warns yang paling tinggi dicerap dalam eksperimen yang menggunakan 1.00% (w/v) NaCl, tetapi aktiviti CMCase didapati rendah.

Key words: Batch culture, cellulase, sodium chloride, Aspergillus terreus SUK-1


Bull, A.T. and Bushell, M.E. 1975. Environmental control of fungal growth. In: Filamentous Fungi: Biosynthesis and Metabolisms. Vol. 2. J.E. Smith & D.R. Berry (eds). Edward Arnold, London, p. 24-26.

Enari, T.M. 1983. Microbial cellulase. In: Microbial Enzyme and Biotechnology. W.M. Fogarty (ed). Applied Science Publication. London, p. 183-223.

Heinz, E., Geek, P. and Wilbrandt, W. 1972, Coupling in secondary active transport: activation of transport by co-transport and/or counter-transport with the fluxes of other solutes Biochimica et Biophisica Acta. 225: 442-462.

Jennings, D.H. 1975. Transport and tranlocation in Filamentous fungi. In: Filamentous Fungi: Biosynthesis and Metabolisms. Vol. 2. J.E. Smith & D.R. Berry (eds.). Edward Arnold, London p. 42-43.

Kelavkar, U., Rao, K.S. and Chhatpar, H.S. 1993 Sodium chloride stress induced morphological and ultrastructural changes in Aspergillus repens. Indian Journal of Experimental Biology, 31: 511-515.

Kelavkar, U., Pandya, D. and Chhatpar, H.S. 1993. Salt stress and respiration in Aspergillus repens. Current Microbiology, 26: 23-29.

Mandels, M., Andreotti, R. and Roche, C. 1976. Measurement of saccharifying cellulase. Biotechnology and Bioengineering Symposium, 6: 21-33.

Parekh, T.V. and Chhatpar, H.S. 1989. Effect of salt stress on the respiratory activity of Aspergillus sydowii. Current Microbiology, 19: 297-301.

Ramos, L.P., Nazhad, M.M. and Saddler, J.N. 1993. Effect of enzymatic hydrolytic on the morphology and fine structure of pretreates cellulosic residues. Enzyme Microbiology Technology, 15: 821-830.

Somogyi, M. 1952. Notes on sugar determination. Journal of Biological Chemistry. 195: 19-23.

Theodorou, M.K., Bazin, M.J. and Trinci, A.P.J. 1981. Growth of Trichoderma reesei and the production of cellulolytic enzymes in a continuous-flow column. Archives Microbiology, 130: 372-380.

Wase, D.A.J. and Vaid, A.K. 1983. Isolation and mutation of highly cellulolytic strain of Aspergillus fumigatus. Process Biochemistry, 22: 217-226.

Wood, T.M. 1985. Properties of cellulolytic enzyme systems. Biochem. Soc. Trans, 13: 407-420.

Wood, T.M and McCrae, S.I. 1979. Synergism between enzymes involved in the solubilization of native cellulose. In: Hydrolysis of Cellulose: Mechanism of Enzymatic and Acid Catalyze. Brown, R.D and Jurasek, C. (eds). Advances in Chemistry Series. 181: 181-209.

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