Malays. Appl. Biol. (1998) 27(1 & 2):
FORMOTHION-INDUCED BIOCHEMICAL CHANGES IN BLOOD AND TISSUES OF FRESHWATER CATFISH, HETEROPNEUSTES FOSSILIS
NARENDRA N. SINGH1* and ANIL K. SRIVASTAVA2
1 Fish Physiology Laboratory, Department of Zoology, St. Andrew's College, Gorakhpur - 273001, U.P., India
2 Department of Zoology, University of Gorakhpur, Gorakhpur - 273009 U.P., India
ABSTRACT
Exposure of the freshwater catfish, Hetempneustes fossilis to a sublethal concentration (4.34 mg/L; one third of 96 hi LC3()) of an organophosphate pesticide formothion, (commercial grade, EC 25%) for 1, 2 and 4 weeks induced market alteration in blood and tissue chemistry. Hyperglycemia along with liver and muscle glycogenolysis, occurred at all time-intervals with the exception of muscle glycogenesis at 1 week. The fishes exhibited a consistent decline in hepatic proteir :ontent. Normal muscle protein values were observed at 1 and 2 weeks but hypoproteinemia in the muscle was noticed al 4 weeks of treatment. Treated catfish exhibited hypocalcemia and hypermagnesemia at 2 and 4 weeks. However, t significant increase in serum inorganic phosphate was recorded 1 and 2 weeks postexposure to formothion.
ABSTRAK
Pendedahan ikan keli air tawar, Hetempneustes fossilis kepada kepekatan subletal (4.34 mg/L; satu pertiga LC50 96 jam) formotion, sejenis pestisid organofosfat (gred komersial, EC 25%) selama 1, 2 atau 4 minggu menghasilkan pertukaran-jertukaran kimia darah dan tisu yang ketara. Hiperglisemia beserta glikogenolisis hati dan otot berlaku selepas setiap angka masa perlakuan, kecuali glikogenesis otot pada 1 minggu. Kandungan protein hepar menurun dengan konsisten. ^ilai protein otot normal dicerap pada 1 dan 2 minggu tetapi hipoproteinemia dicerap selepas 4 minggu perlakuan. Ikan fang diberi perlakuan mempamerkan hipokalemia dan hipermagnesemia pada 2 dan 4 minggu. Walau bagaimanapun, cenaikan signifikan aras fosfat tak organik serum direkodkan pada 1 dan 2 minggu pasca pendedahan kepada formotion.
Key words: Carbohydrate, electrolytes, formothion, protein, teleost, toxicity
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