Malays. Appl. Biol. (1998) 27(1 & 2): 45-49
EFFECTS OF ACTIVE MOLLUSCICIDAL AGENTS OF COMMON SPICES ON BIOCHEMICAL PARAMETERS IN THE OVOTESTIS OF LYMNAEA ACUMINATA
SANJAY SINGH, V. K. SINGH and D. K. SINGH*
Department of Zoology, DDU Gorakhpur University Gomkhpur-273 009, India
ABSTRACT
The effects of active molluscicidal components of Zingiber officinale (citral and [6]-gingerol, respectively) and Trachyspermum ammi (thymol) on total protein, free amino acids and nucleic acid levels, and phosphatase activities were studied in the ovotestis of L. acuminata. Sublethal exposure to these components caused significant alterations in these biochemical parameters. Maximum reduction in protein levels of ovotestis (35% of control) was observed in snails exposed for 96 hr to 80% of 24 hr LC50 of [6]-gingerol. Significant reduction in amino acid levels was observed in ovotestis of [6]-gingerol- and thymol-treated snails whereas a significant increase was observed in citral treated snails. Significant reduction in nucleic acid level and acid/alkaline phosphatase activity in ovotestis were observed in all three groups of treated snails. Withdrawal experiments showed that these changes were reversible.
ABSTRAK
Kesan komponen-komponen moluskisid aktif dari Zingiber officinale (sitral dan [6]-gingerol, masing-masing) dan Trachyspermum ammi (timol) ke atas protein keseluruhan, aras asid amino bebas dan asid nukleik, serta aktiviti protease ;elah dikaji daiam ovotestis L. acuminata. Pendedahan subletal terhadap bahan-bahan ini menyebabkan pertukaran signifikan ke atas parameter-parameter biokimia tersebut. Pengurangan maksimum aras protein dalam ovotestis (35% dari kawalan) Jicerap dalam haiwan yang terdedah selama 96 jam kepada 80% LC50 24 jam [6]-gingerol. Pengurangan signifikan aras asid amino dicerap dalam ovotestis haiwan terdedah kepada [6]-gingerol dan timol. Dalam haiwan yang terdedah kepada iitral aras asid amino meningkat dengan signifikan. Dalam ketiga-tiga kumpulan haiwan pengurangan signifikan aras asid mkleik dan aktiviti asid/alkalin fosfatase dicerap. Kesan-kesan ini adalah berbalik.
Key words: Citral, [6]-gingerol, molluscicide, spices, thymol
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