Malays. Appl. Biol. (1998) 27(1 & 2): 91-99
COMPARISON OF LIGNIN DEGRADING ENZYME PRODUCTIVITY BY FREE AND IMMOBILIZED CELLS OF PHANEROCHAETE CHRYSOSPORIUM IN AGITATED CULTIVATION VESSEL
DARAH,I. and IBRAHIM, C.O.*
Fermentation and Enzyme Technology Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
ABSTRACT
The production of lignin degrading enzymes, namely lignin peroxidase (LiP) and manganese peroxidase (MnP), by free and entrapped cells of Phanerochaete chrysosporium in scouring meshes and calcium alginate beads using a stirred tank bioreactor system was studied. The results revealed that a high productivity of 14.62 mU/gm/day was observed for LiP using scouring mesh entrapped cells. However, free cells and Ca-alginate entrapped cells showed lower productivities of 10.83 and 11.36 mU/gm/day, respectively. The productivity for MnP was also higher using the scouring mesh entrapped cells. The high productivity afforded by the scouring mesh entrapped cells was due to the protective effect of the support materials from agitational stress, and the large cavity size of the material which enhanced fungal growth.
ABSTRAK
Penghasilan enzim pengurai lignin, iaitu lignin peroksidase (LiP) dan mangan peroksidase (MnP), oleh sel Phanerochaete chrysosporium yang bebas dan terperangkap di dalam gabus dan bintilan kalsium alginat menggunakan sistem bioreaktor tangkai teraduk dikaji. Hasil kajian menunjukkan daya pengeluaran LiP setinggi 14.62 mU/gm/hari didapati oleh sel yang terperangkap di dalam gabus. Walau bagaimanapun, sel bebas dan sel terperangkap di dalam Ca-alginat, menunjukkan daya pengeluaran yang lebih rendah, iaitu masing-masing, 10.83 dan 11.36 mU/gm/hari. Daya pengeluaran MnP juga didapati lebih tinggi menggunakan sel yang terperangkap di dalam gabus. Daya pengeluaran tinggi yang ditunjukkan oleh sel terperangkap di dalam gabus adalah disebabkan oleh kesan perlindungan daripada tekanan pengadukan oleh bahan pembawa dan juga saiz rongga bahan pembawa yang besar yang dapat meningkatkan pertumbuhan kulat.
Key words: Lignin degrading enzyme production, lignin peroxidase, immobilized cells, manganese peroxidase, stirred tank bioreactor
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