Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2008) 37(1):41–46



Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur, 273 009, U.P. India *E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


The toxic effect of dried berries powder of Piper cubeba, dried fruit powder of Piper longum and Tribulus terrestris against snail Lymnaea acuminata was studied. Toxicity of these plant products were time and concentration dependent. The toxicity of Piper longum fruit powder (96h LC50- 48.99 mg/l) was more effective than fruit powder of Piper cubeba (96h LC50- 54.01 mg/l) and fruit powder of Tribulus terrestris (96h LC50- 83.49 mg/l). Ethanol extracts of these plants were more effective than other organic extracts. 96h LC50 of column purified fraction of Piper cubeba against L. acuminata was 3.57 mg/l, whereas 96h LC50 of the column purified fraction of Piper longum and Tribulus terrestris was 5.03 mg/l and 13.53 mg/l, respectively. These plants may be used as potent source of molluscicide against the snail Lymnaea acuminata.

Key words: Lymnaea acuminata, Fascioliasis, cubebene, piperine, harmane, harmine


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