Malays. Appl. Biol. (2008) 37(1): 57–62
OPTIMAL CYTOCHALASIN B FOR INDUCTION OF TRIPLOIDY IN TROPICAL OYSTER, CRASSOSTREA IREDALEI (FAUSTINO) EMBRYOS AND THEIR SHORT-TERM SURVIVAL RATE
MD-SALEH, M.T.1, MASAZURAH, A.R.2 and DEVAKIE, M.N.2*
1 National Prawn Fry Production and Research Centre (NAPFRE), Pulau Sayak, Kedah, Malaysia
2 Fisheries Research Institute (FRI), Batu Maung, 11960, Penang, Malaysia
*E-mail:
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ABSTRACT
A study was undertaken to determine the optimal Cytochalasin B (CB) concentration to induce triploidy in the tropical oyster, Crassostrea iredalei so that commercial production of triploids could be carried out. Three sets of triploid induction trials using the chemical, CB were carried out on strip-spawned eggs of the tropical oyster. The effect of four concentrations (0.25 ppm; 0.5 ppm; 0.75 ppm and 1.0 ppm) in inducing triploidy was evaluated. An untreated batch was kept as the control (0.0 ppm) for each trial. The short term survival rate of the oyster larvae up to 48 hrs was also studied. The one-way ANOVA indicated a significant difference at P < 0.05 in the triploidy levels for the various concentrations of CB used. Higher triploid yields of 51.3 ± 6.9% and 51.2 ± 6.7% were obtained for concentrations 0.75 ppm and 1.0 ppm, respectively which were not significantly different (P > 0.05) from each other. The short term survival rate of the larvae from different CB concentrations and the control had also indicated a significant difference at P < 0.05. The highest survival rate was recorded for the control at 0.0 ppm (51.7 ± 27.3%) while survival rates ranging from 7–20% for all other concentrations were not significantly different P > 0.05 from each other.
ABSTRAK
Kajian dijalankan untuk mengenalpasti kepekatan optimal Cytochalasin B (CB) yang diperlukan untuk mengaruhkan keadaan triploid pada tiram tropika, Crassostrea iredalei, untuk meghasilkan tiram triploid secara komersial. Tiga set percubaan aruhan triploid menggunakan CB dilakukan ke atas telur tiram yang dihasilkan dengan memicit keluar telurnya. Kesan ke atas empat kepekatan (0.25 ppm; 0.5 ppm; 0.75 ppm and 1.0 ppm) dinilai. Kelompok yang tidak dirawat (0ppm) dijadikan sebagai kawalan bagi setiap percubaan. Kadar kemandirian sehingga 48 jam setelah persenyawaan turut dikaji. Ujian ANOVA sehala menunjukkan perbezaan yang signifikan pada P<0.05 bagi paras triploid untuk semua kepekatan CB yang digunakan. Kajian telah menunjukkan kepekatan CB pada 0.75 ppm dan 1.0 ppm menghasilkan peratusan triploid yang tinggi, masing-masing 51.3 + 6.9% dan 51.2 + 6.7% dan didapati tiada perbezaan yang signifikan (P>0.05) antara kedua kepekatan tersebut. Kadar kemandirian jangka pendek bagi larva dari kepekatan CB yang berbeza dan kawalan juga menunjukkan perbezaan yang signifikan pada P<0.05. Kadar kemandirian yang paling tinggi direkodkan untuk kawalan pada 0.0 ppm (51.7 + 27.3%) sementara kepekatan yang lain menunjukkan prestasi antara 7-20% di mana tiada perbezaan signifikan antara satu sama lain.
Key words: tropical oyster embryos, Crassostrea iredalei, Cytochalasin-B, triploidy, survival rate
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