Malays. Appl. Biol. (2006) 35(1): 7–13
CRYSTALLINE STYLE AND BYSSUS OF PERNA VIRIDIS AS INDICATORS OF NI BIOAVAILABILITIES AND CONTAMINATION IN COASTAL WATERS OF PENINSULAR MALAYSIA
YAP*, C.K., ISMAIL, A., CHENG, W.H., EDWARD, F.B. and TAN, S.G.
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
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ABSTRACT
The concentrations of Ni in eight different soft tissues of the green-lipped mussel Perna viridis from 6 geographical sites along the coastal waters off Peninsular Malaysia were determined. The crystalline style (CS) and byssus, when compared with mantle, gills, muscle, gonad, foot, and the remaining soft tissue, accumulated significantly (at least P< 0.05) higher concentrations of Ni in both contaminated and uncontaminated samples, indicating that the style and byssus have a higher affinity for Ni to bind with metallothioneins. The higher concentrations of the Ni in the CS and byssus than in the other soft tissues became significantly (P< 0.05) higher (more pronounced) in mussels collected from contaminated coastal waters in the Johore Strait. This indicated a tissue redistribution of Ni due to its binding to metallothioneins for Ni detoxification. Therefore, the potential of CS and byssus as indicators of Ni bioavailability and contamination is suggested since the metal levels in them significantly and positively correlated with the Ni pollution levels represented by the Ni concentrations in the surface sediments of the sampling sites.
ABSTRAK
Kepekatan Ni di dalam lapan tisu yang berlainan bagi kupang Perna viridis daripada enam lokasi di persisiran pantai Semenanjung Malaysia telah ditentukan. Stil berhablur (CS) dan bisus, apabila dibandingkan dengan mantel, insang, otot, gonad, kaki dan tinggalan tisu lembut, menimbun lebih tinggi kepekatan Ni secara signifikan (sekurang- kurangnya P< 0.05) di kedua-dua kawasan tercemar dan tidak tercemar. Ini menunjukkan bahawa CS dan bisus tersebut mempunyai affiniti yang lebih tinggi untuk Ni melekat dengan metallothionein. Kepekatan Ni yang lebih tinggi di dalam CS dan bisus daripada tisu lembut yang lain menjadi lebih tinggi secara siginifikan (P< 0.05) [lebih jelas tinggi kepekatannya] di dalam kupang tercemar yang disampel daripada Selat Johor. Ini menunjukkan bahawa taburan semula tisu bagi Ni disebabkan oleh lekatan dengan metallothionein untuk detoksifikasi Ni. Oleh itu, kajian ini mencadangkan potensi CS dan bisus sebagai penunjuk bio-tersediaan dan pencemaran bagi Ni kerana korelasi bagi kepekatan Ni pada kedua-dua tisu ini adalah secara positif dan signifikan dengan pencemaran Ni yang ditunjukkan oleh kepekatan Ni di dalam sedimen di kawasan persampelan.
Key words: Ni, Perna viridis, tissue distribution
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