Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

37-2-04

E-mail Print PDF
Malays. Appl. Biol. (2008) 37(2): 25–28

SEX DETERMINATION OF Pistacia vera L. USING ISSR MARKERS

ALI AKBAR EHSANPOUR*, MANOOCHEHR TAVASSOLI and LILA ARAB

Dept. of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

ABSTRACT

Gender of four female and male individual Pistacia vera cultivars (Akbari, Ahmad Aghaii, Fandoghi, Kaleh Ghochi) were identified using nine Inter Simple Sequence Repeat (ISSR) primers. Two primers (AC)8CG and (AC)8TA were able to identify male and female plants by producing sex dependent DNA bands in female plants. PCR condition was reproducible and reliable for sex determination of Pistacia cultivars. It can be recommended for sex determination of Pistacia plants at early stage of seedling growth.

Key words: Pistacia vera, sex determination, ISSR

REFERRENCES

Albani, M.C., Battey, N.H. and Wilkinson, M.J. 2004. The development of ISSR-derived SCAR markers around the Seasonal Flowering Locus (SFL) in Fragaria vesca. Theor. Appl. Genet., 109: 571–579.

Doyle, J.J., and Doyle, J.L. 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19: 11-15.

Epplen, J.T. 1982. Base sequence of a cloned snake W-chromosome DNA fragment and identification of a male-specific putative mRNA in the mouse. Proc. Natl. Acad. Sci., 79: 3798–3802.

Irish, E.E. and Nelson, T. 1989. Sex determination in monoecious and dioecious plants. Plant Cell, 1: 737-744.

Karp, A., Seberg, O. and Buiatti, M.M. 1996. lecular techniques in the assessment of botanical diversity. Annals of Botany, 78: 143– 149.

Lanham, P.G. and Brennan, R.M. 1999. Genetic characterization of gooseberry (Ribes grossularia subgenus Grossularia) germplasm using RAPD, ISSR and AFLP markers. J Hort Sci Biotech., 74: 361–366.

Goulão, L. and Olivera, C.M. 2001. Molecular characterisation of cultivars of apple (Malus × domestica Borkh.) using microsatellite (SSR and ISSR) markers. Euphytica, 122: 81–89.

Nanda, I. 1992. Early stages of sex-chromosome differentiation in fish as analysed by simple repetitive DNA sequences. Chromosoma, 101: 301–310.

Navinder, S., Neelu, J., Sunita, J. and Rajinder, K.J. 2004. Assessment of genetic diversity within and among Basmati and non-Basmati rice varieties using AFLP, ISSR and SSR markers. Euphytica, 140: 133–146.

Parasnis, A.S., Ramakrishna, Chowdari, W.K.V., Gupta, V.S. and Ranjekar, P.K. 1999. Microsatellite (GATA)n reveals sex-specific differences in Papaya. Theor. Appl. Genet., 99: 1047–1052.

Parker, P.G., Snow, A.A., Schug, M.D., Booton, G.C. and Fuerst, P.A. 1998. What molecule can tell us about populations: Choosing and using a molecular marker. Ecology, 79: 361– 382.

Ramakrishna, W. 1995. DNA fingerprinting to detect genetic variation in rice using hypervariable DNA sequences. Theor. Appl. Genet., 90: 1000–1006.

Schafer, R. 1986. The expression of the evolutionarily conserved GATA/GACA repeats in mouse tissues. Chromosoma, 93: 496–501.

Wannan, B.S. and Quinn, C.J. 1991. Floral structure and evolution in the Anacardiaceae. Bot. J. Linn. Soc., 107: 349-385.

Wolfe, A.D. and Liston, A. 1998. Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Plant Molecular Systematics (II Eds.) D.E. Soltis, P.S. Soltis and J.J. Doyle., pp. 43-86. Kluwer.

Wu, K.S. and Tanklsley, S.D. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet., 241: 225–235.

YAO Bing, H.U., Xiaoli, B.A.O., Zhenmin, L.U., Wei, H.U. 2007. Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers. Chinese Journal of Oceanology and Limnology, 25: 91-96.

Zietkiewicz, E., Rafalski, A. and Labuda, D. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 20: 176–183.
 

Main Menu