Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2008) 37(2): 25–28



Dept. of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
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Gender of four female and male individual Pistacia vera cultivars (Akbari, Ahmad Aghaii, Fandoghi, Kaleh Ghochi) were identified using nine Inter Simple Sequence Repeat (ISSR) primers. Two primers (AC)8CG and (AC)8TA were able to identify male and female plants by producing sex dependent DNA bands in female plants. PCR condition was reproducible and reliable for sex determination of Pistacia cultivars. It can be recommended for sex determination of Pistacia plants at early stage of seedling growth.

Key words: Pistacia vera, sex determination, ISSR


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