Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2007) 36(1): 23–29


SIRAJ, S.S., ESA, Y.B., KEONG, B.P. and DAUD, S.K.

Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
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Randomly Amplified Polymorphic DNA (RAPD) marker was used to examine the genetic relationships among three populations of two different colour-types (silver-bronze and reddish) of ikan kelah (Tor tambroides). Sixty three individuals of the kelah were sampled from Sia River of Pahang and Kampung Esok River of Negeri Sembilan (silver- bronze) and Nenggiri River of Kelantan (reddish). Twelve RAPD primers generated a total of 226 scorable loci with 100% polymorphism across the sixty-three individuals. The RAPD banding patterns and sizes ranged from 4 to 17 and from 100bp to 1500bp, respectively. The intra-population UPGMA dendrogram produced two major clusters, with the Nenggiri River (Kelantan) samples formed a subcluster in both major clusters dominated by the Pahang samples (Cluster 1) and N. Sembilan (Cluster 2) samples, respectively. The inter-population UPGMA showed that the Kelantan samples were genetically closer to the N. Sembilan samples than to the Pahang samples. Thus, the results of this study did not support the hypothesis that the two colour-types of T. tambroides were genetically distinct from each other.


Penanda ‘Randomly Amplified Polymorphic DNA (RAPD)’ telah digunakan untuk melihat hubungan genetik di kalangan tiga populasi ikan kelah (Tor tambroides) yang mempunyai dua jenis warna berbeza (perak-gangsa dan kemerahan). Enam puluh tiga individu kelah telah disampel dari Sungai Sia, Pahang dan Sungai Kampung Esok, Negeri Sembilan (jenis warna perak-gangsa) dan Sungai Nenggiri, Kelantan (jenis warna kemerahan). Dua belas “primer” RAPD yang telah dipilih menghasilkan sejumlah 226 lokus yang 100% polimorfik daripada enam puluh tiga individu tersebut. Corak dan saiz lokus RAPD yang terhasil daripada ketiga-tiga populasi berjulat daripada 4 hingga 17 lokus, dan daripada 100bp hingga 1500bp, masing-masing. Keputusan dendrogram UPGMA intra-populasi menghasilkan dua kelompok utama; sampel-sampel dari Sungai Nenggiri (Kelantan) membentuk subkelompok di dalam kedua-dua kelompok utama yang didominasi oleh masing-masing sampel-sampel dari Pahang (Kelompok 1) dan N. Sembilan (Kelompok 2). Keputusan dendrogram UPGMA untuk inter-populasi menunjukkan sampel-sampel dari Kelantan adalah lebih dekat secara genetik dengan sampel-sampel dari N. Sembilan berbanding sampel-sampel dari Pahang. Justeru, keputusan daripada kajian ini tidak menyokong hipotesis bahawa kedua-dua jenis warna T. tambroides adalah berbeza secara genetik di antara satu sama lain.

Key words: Freshwater fish, Tor tambroides, colour-type, RAPD, genetic marker


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