Malays. Appl. Biol. (2011) 40(2): 43–50
DETECTION OF GENETIC STRUCTURE AMONG RAMIN (Gonystylus bancanus (Miq.) Kurz) POPULATIONS IN PENINSULAR MALAYSIA USING A RAPID DNA FINGERPRINTING TECHNIQUE
FATMA, N.A.H.1,2, WICKNESWARI, R.1* and CANNON, C.H.3,4
1School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
2International Education College, Universiti Teknologi MARA, 40200 Shah Alam, Selangor, Malaysia.
3Texas Tech University, Dept. of Biological Sciences, Box 43131, Lubbock, TX 79409-3131, United States of America.
4Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Yunnan 666303, China.
*Email:
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ABSTRACT
Here, we demonstrate the power of DNA fingerprints, generated by Direct Amplification of Length Polymorphism (DALP) analysis to understand the genetic structure and variation of five natural populations of ramin (Gonystylus bancanus) in Peninsular Malaysia. Six primer sets generated 309 distinct fragments ranging from 100-1200 bp among 156 individuals. These loci were highly polymorphic (83%) and no two individuals shared the exact same fingerprint. Most of the genetic variation was found within populations (only 13% among population variance) although cluster analysis indicated that most individuals could be correctly assigned to their original population. Genetic similarity among populations was not correlated with geographic distance, possibly due to environmental differences among the sampled peat forests. The DNA fingerprinting approach presented here would be highly effective for tracking individual logs from forest to market and detecting illegal smuggling. These DNA fingerprints could also be applied for correct deployment of seedlings in enrichment planting schemes to avoid breakdown of co-adapted complexes for survival and growth in the extreme peat swamp environment.
Key words: Genetic structure, genetic variation, genetic relatedness, DALP analysis, Gonystylus bancanus (ramin), conservation, sustainable management
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