Malays. Appl. Biol. 41(1): 23–28
ANTIOXIDANTS ACTIVITY IN PINEAPPLE CV. N36 CULTURE UNDER ALUMINIUM STRESS
AZIZ, A.* and NURHALIM, M.S.
Department of Biological Sciences, Faculty Science and Technology University,
Malaysia Terengganu Kuala Terengganu, Terengganu, 21030 Malaysia.
*Email:
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; Phone: 609-6684165; Fax: 6096684167
ABSTRACT
Aluminium is an important stress factor for plants in acidic environments. In the present study, the effect of aluminium-stress on the oxidative enzymes and antioxidant activities in pineapple cv. N36 plantlets was investigated. The plantlets were cultured in MS medium containing 100 µM Al (pH 4.0) for 30 days. Results showed that fresh and dry weight, ascorbic acid content and catalase (CAT) enzyme activity were not significantly (p>0.05) affected by Al-stress. Al did significantly (p<0.05) reduce the alpha-tocopherol content (2.76 mg/fwt.) and guaiacol peroxidase (GPX) enzyme activity (3.39 units/mg protein), and increased the enzyme ascorbate peroxidase (APX) activity (7.16 units/mg protein). These results indicated that long-term Al-stress did change antioxidant substances content and oxidative enzymes activities in the leaves of pineapple cv. N36 plantlets.
ABSTRAK
Aluminium merupakan faktor tekanan utama bagi tumbuhan di kawasan berasid. Dalam kajian ini, kesan tekanan-aluminum ke atas enzim oksidatif dan aktiviti aktioksidan dalam Ananas cv. N36 telah dikaji. Anak benih telah dikultur dalam medium MS mengandungi 100 µM Al (pH 4.0) selama 30 hari. Keputusan menunjukkan berat basah dan kering, kandungan asid askorbik dan aktiviti enzim katalase (CAT) tidak dipengaruhi (p>0.05) oleh tekanan Al. Al memberi kesan yang bererti (p<0.05) terhadap penurunan kandungan tokoferol (2.76 mg/berat basah) dan aktiviti enzim guaikol peroksidase (GPX) (3.39 unit/mg protein), dan peningkatan aktiviti enzim askorbat peroksidase (APX) (7.16 unit/mg protein). Keputusan ini menunjukkan tekanan jangka panjang Al telah mengubah kandungan sebatian antioksidan dan aktiviti enzim oksidatif dalam daun-daun anak pokok Ananas cv. N36.
Key words: catalase, peroxidase, ascorbate peroxidase, carotenoids, ascorbic acid, alpha-tocopherol
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