Malays. Appl. Biol. (2014) 43(1): 13–20
MORPHOLOGICAL AND GENETIC VARIABILITIES OF
Fusarium species ISOLATED FROM KENAF
NUR AIN IZZATI, M.Z.*, MOHD AIZAT, Z., SITI NORDAHLIAWATE, M.S. and MAT RASID, I.
Biology Department, Faculty of Science, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia.
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Telephone: +603-89466642
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ABSTRACT
The objectives of this study were to identify the Fusarium species associated with kenaf based on morphological, pathological and genetic characteristics. Twenty isolates of Fusarium were obtained from root, leaf and stem of kenaf in Kuala Terengganu, Malaysia. All Fusarium isolates were identified, and classified into four species such as F. oxysporum, F. proliferatum, F. semitectum and F. solani. Based on pathogenicity test, F. oxysporum was only pathogenic and caused Fusarium wilt disease in kenaf based on disease severity index (DSI), plant height and dry weight of plant. For vegetative compatibility groups (VCGs) study, 217 nitrate non-utilizing (nit) mutants were generated with nit1 was the highest mutants recovered. All the species are genetically diverse reflected from the VCG multiple groups. F. oxysporum isolates were grouped into 5 VCGs, F. proliferatum in 3 VCGs and F. semitectum in 4 VCGs. Fusarium species isolated from kenaf were diverse based on morphology and vegetative compatibility, however only F. oxysporum caused Fusarium wilt disease.
Key words: disease severity index, F. oxysporum, F. proliferatum, F. semitectum, F. solani, vegetative compatibility groups
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