Malays. Appl. Biol. (2014) 43(1): 73–80
ANTI-PLASMODIAL ACTIVITY OF ENGELETIN ISOLATED
FROM Artocarpus scortechinii
ALI, A.H.a, HASSAN, N.M.b, SHUKOR, N.I.b, EMBI, N.a, LATIP, J.b
& MOHD SIDEK, H.M.a*
aSchool of Biosciences and Biotechnology Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor
bSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor
Tel: +603-89215954
*Email:
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
ABSTRACT
Ethnomedicinal use of plants from the Artocarpus genus has been extensively documented to include treatment of inflammation and malarial fever. Anti-plasmodial effects of Artocarpus scortechinii (synonym A. elasticus) reported against P. falciparum 3D7 culture in erythrocytes remain to be validated in vivo. The present study involved re-evaluation of anti-plasmodial activity of ethanolic extract of A. scortechinii in vitro, assessment of the extract for chemo-suppressive effects in vivo, and fractionation and identification of bioactive compounds. The ethanolic extract of A. scortechinii displayed moderate inhibitory activity towards growth of P. falciparum 3D7 (IC50=14.4±8.1 ug/mL) with minimal toxicity effect in mammalian Chang liver cells (selectivity index, SI=13.9). Administration of 25, 50 and 100 mg/kg body weight extract into P. berghei-infected mice for four consecutive days resulted in dose-dependent chemo suppression of 43.2, 74.8 and 78.4% respectively. Vacuum liquid chromatography fractions of the ethanolic extract exhibited moderate to good in vitro anti-plasmodial activities. Further fractionation using Sephadex Column Chromatography yielded engeletin identified by nuclear magnetic resonance spectroscopy and mass spectroscopy. Upon further testing the isolated engeletin was shown to display an IC50 of 33.1±1.3 uM against the 3D7 parasite strain (SI=3.5). Data from the present study is the first report on anti-plasmodial activity of engeletin and its isolation from Artocarpus scortechinii.
Key words: Anti-plasmodial, Artocarpus sp., flavonoid, engeletin
REFERENCES
Bewick, V., Cheek, L. & Ball, J. 2004. Statistics Review 12: Survival Analysis. Critical Care London, 8: 389-394.
Chen, L., Yin, H., Lan, Z., Ma, S., Zhang, C., Yang, Z., Li, P. & Lin, B. 2011. Anti-hyperuricemic and nephroprotective effect of Smilax china L. Journal of Ethnopharmacology, 135 (2): 399-405.
Gathirwa, J. W., Rukunga, G. M., Njagi, E. N. M., Omar, S. A., Guantai, A. N., Muthaura, C. N., Mwitari, P. G., Kimani, C. W., Kirira, P. G., Tolo, F. M., Ndunda, T. N. & Ndiege, I. O. 2007. In vitro anti-plasmodial and in vivo anti-malarial activity of some plants traditionally used for the treatment of malaria by the Meru community in Kenya. Journal of Natural Medicine, 61: 261-268.
Hakim, E. H., Achmad, S. A., Juliawaty, L. D., Makmur, L., Syah, Y. M., Aimi, N., Kitajima, M., Takayama, H. & Ghisalberti, E. L. 2006. Prenylated flavonoids and related compounds of the Indonesian Artocarpus (moraceae). Journal of Natural Medicine, 60 (3): 161–184.
Huang, H., Cheng, Z., Shi, H., Xin, W., Wang, T. T. Y. & Yu, L. 2011. Isolation and characterization of two flavonoids, engeletin and astilbin, from the leaves of Engelhardia roxburghiana and their potential anti-inflammatory properties. Journal of Agricultural and Food Chemistry, 59 (9): 4562-4569.
Itharat, A., Ruangnoo, S., Makchuchit, S., Thongdeeying, P. & Panthong, S. 2012. Anti-inflammatory compounds of Smilax corbularia Kunth. Planta Medica, 78 (11): 148-149.
Jagtap, U. B. & Bapat, V. A. 2010. Artocarpus: a review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology, 129 (2): 142–166.
Jayasinghe, L., Balasooriya, B. A. I. S., Padmini, W. C., Hara, N. & Fujimoto, Y. 2004. Geranyl chalcone derivatives with anti-fungal and radical scavenging properties from the leaves of Artocarpus nobilis. Phytochemistry, 65 (9):1287–1290.
Khan, M. R., Omoloso, A. D. & Kihara, M. 2003. Anti-bacterial activity of Artocarpus heterophyllus. Fitoterapia, 74 (5): 501–505.
Ko, H. H., Lu, Y. H., Yang, S. Z., Won, S. J., & Lin, C. N. 2005. Cytotoxic prenylflavonoids from Artocarpus elasticus. Journal of Natural Products, 68 (11): 1692–1695.
Mabry, T. J., Markham K. R. & Thomas, M. B., 1970. The Systematic Identification of Flavonoids. New York: Springer Verlag.
Makler, M. T. & D. J. Hinrichs. 1993. Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitaemia. Journal of Tropical Biomedicine, 48 (2): 205-210.
McIntyre, D. D., Germann M., W. & Vogel, H. J. 1989. Conformational analysis and complete assignment of the proton and carbon NMR spectra of ouabain and ouabagenin. Canadian Journal of Chemistry, 68 (8): 1263-1270.
Mossmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunology, 65 (1): 55–63.
Mustapha, I., Hakim, E. H., Juliawaty, L. D., Syah, Y. M. & Achmad, S. A. 2010. Prenylated flavones from some Indonesian Artocarpus and their anti-malarial properties. Medicinal Plants-International Journal of Phyto-medicines and Related Industries, 2 (2): 157-160.
Nkhoma, S., Molyneux, M. & Ward, S. 2007. In vitro anti-malarial susceptibility profile and prcrt/pfmdr-1 genotypes of Plasmodium falciparum field isolates from Malawi. American Journal Tropical Medicinal Hygiene, 76 (6): 1107-1112.
Peters, W. 1975. The chemotherapy of rodent malaria, XXII. The value of drug-resistant strains of P. berghei in screening for blood schizontocidal activity. Annals of Tropical Medicine and Parasitology, 69 (2): 155-171.
Rasoanaivo, P., Ramanitrahasimbola, D., Rafatro, H., Rakotondramanana, D., Robijaona, B., Rakotozafy, A., Ratsimamanga?Urverg, S., Labaïed, M., Grellier, P. & Allorge, L. 2004. Screening extracts of Madagascan plants in search of anti-plasmodial compounds. Phytotherapy Research, 18 (9):742-747.Ruangnoo, S., Jaiaree, N., Makchuchit, S., Panthong, S., Thongdeeying, P. & Itharat, A. 2012. An in vitro inhibitory effect on RAW 264.7 cells by anti-inflammatory compounds from Smilax corbularia Kunth. Asian Pacific Journal of Allergy and Immunology, 30 (4): 268.
Tanamatayarat, P., Sotanaphun, U. & Poobrasert, O. 2000. Thai plants from Doi Tung: brine shrimp lethality, anti-oxidative activity and combination effect with L-ascorbic acid. Natural Product Research, 26 (10): 919-925.
Trager, W. & Jensen, J. B. 1976. Human malaria parasites in continuous culture. Science, 193 (4254): 673-675.
Weng, J. R., Chan, S. C., Lu, Y. H., Lin, H. C., Ko, H. H. & Lin, C. N. 2006. Anti-platelet prenylflavonoids from Artocarpus communis. Phytochemistry, 67 (8): 824–829.
Xu, J., Li, X., Zhang, P., Li, Z. L. & Wang U. 2005. Anti-inflammatory constituents from the roots of Smilax bockii. Archives of Pharmacal Research, 28 (4): 395-399.
