Malays. Appl. Biol. (2014) 43(1): 133–139
ALISISIS AKTIVITI ENZIM DAN ELISA KE ATAS PENANDA
BIOLOGI PERGERAKAN GIGI DALAM CECAIR KREVIS
GINGIVA MANUSIA SEMASA RAWATAN ORTODONTIK
NURFATHIHA, A.K.1, ROHAYA, M.A.W.2*, SAHIDAN, S.1, ABDUL AZIZ, J.3, INTAN ZARINA, Z.A.1,
ZAIDAH, Z.A.4 dan SHAHRUL HISHAM, Z.A.1
1Pusat Pengajian Biosains dan Bioteknologi, Fakulti Sains dan Teknologi,
Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
2*Jabatan Ortodontik, Fakulti Pergigian, Universiti Kebangsaan Malaysia,
50300 Kuala Lumpur, Malaysia
3DELTA, Pusat Pengajian Matematik, Fakulti Sains dan Teknologi,
Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
4Department of Microbiology, Faculty of Applied Science, Universiti Teknologi MARA,
40450, Shah Alam, Selangor, Malaysia
*E-mel:
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ABSTRACT
CurrentTujuan kajian ini adalah untuk mengenalpasti perhubungan diantara aktiviti spesifik laktat dehidrogenase (LDH), aspartat aminotransferase (AST), asid fosfatase rintang tartrat (TRAP) dan alkali fosfatase (ALP) dengan jumlah enzim masing-masing yang hadir dalam cecair krevis gingiva (gingiva crevicular fluid; GCF) semasa rawatan ortodontik dengan menggunakan analisis statistik. Sampel GCF diambil daripada 19 orang subjek ortodontik pada masa-masa tertentu; sebelum memakai pendakap gigi (basal) dan selepas memakai pendakap gigi [minggu 0 (sebelum dikenakan tekanan) dan minggu 1 hingga minggu 5 (selepas daya diberikan)]. Subjek menerima daya 1.0 N dan 1.5 N sama ada di bahagian kanan atau kiri arkus maksila. Pengasaian enzim dan analisis ELISA dilakukan. Analisis korelasi Pearson menunjukkan tiada korelasi yang tinggi dan signifikan (p>0.05) di antara jumlah enzim dengan aktiviti spesifik enzim semasa rawatan ortodontik pada kedua-dua daya (1.0 N dan 1.5 N) bagi kesemua enzim penanda yang dikaji; LDH (1.0 N, r=-0.28, p>0.05; 1.5 N, r=-0.08, p>0.05), AST (1.0 N, r=-0.49, p>0.05; 1.5 N, r=0.08, p>0.05), TRAP (1.0 N, r=0.016, p>0.05; 1.5 N, r=0.48, p>0.05) dan ALP (1.0 N, r=-0.28, p>0.05; 1.5 N, r=-0.08, p>0.05). Oleh itu, asai enzim lebih sesuai digunakan berbanding analisis ELISA untuk melihat profil aktiviti enzim penanda yang aktif semasa rawatan ortodontik berdasarkan kepada proses pergerakan gigi yang melibatkan enzim aktif.
Key words: Laktat dehidrogenase (LDH), aspartat aminotransferase (AST), asid fosfatase rintang tartrat (TRAP) dan alkali fosfatase (ALP)
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