Malays. Appl. Biol. (2014) 43(2): 35-40
SURVIVIN EXPRESSION IN COLORECTAL: REAL TIME POLYMERASE CHAIN REACTION AND CORRELATION WITH CLINICOPATHOLOGICAL FEATURES.
SAID, S.1, ALFRED-KING, Y.L.2 and YIK-HONG, H.
1Discipline of Surgery (School of Medicine) and North Queensland Centre for Cancer Research (Australian Institute of Tropical Medicine), James Cook University, Townsville, Queensland 4811, Australia.
2 Discipline of Pathology, Queensland Health & Pathology Scientific Services, Gold Coast Hospital and Griffith Medical School, Gold Coast, Queensland 4215, Australia.
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ABSTRACT
Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy.
Keywords
Survivin, Colorectal cancer, Real time PCR
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