Malaysian Applied Biology Journal

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44_1_19

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Malays. Appl. Biol. (2015) 44(1): 115-118

 

PRELIMINARY STUDY OF IN VITRO PROPAGATION OF A SELECTED HIGH YIELDING CLONE OF KACIP FATIMAH (Labisia pumila VAR. alata)

SYAFIQAH NABILAH,S.B.1,3*, FARAH FAZWA, M.A.1, NOR HASNIDA, H.2, SITI SUHAILA, A.R.2, NORHAYATI, S.1, NAZIRAH, A.2, MUHAMMAD FUAD, Y.2, HALIZA, I.2 and MOHAMAD, O.3

1 Plant Improvement Programme, Forest Biotechnology Division,

Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia.

2Tissue Culture Laboratory, Forest Biotechnology Division,

Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia.

3Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA,

40450 Shah Alam, Selangor, Malaysia.

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

ABSTRACT

Kacip Fatimah (Labisia pumila), an undershrub herb from the Myrsinaceae family, is traditionally used as medicine, particularly by Malay women during post-natal care. The development of L. pumila as a medicinal herb has led to its commercialization and increasing demand. To ensure its sustainable supply, the propagation of kacip Fatimah on a large scale such as through tissue culture needs to be explored. In this study, the leaves of a selected high yielding clone of kacip Fatimah, clone AA28, were used as explants. After surface sterilization, the explants were cultured on MS basal medium containing 0.5 mg/l NAA with different concentrations of BAP ranging from 0.05 to 0.15 mg/l for shoot induction. The explants cultured in MS medium containing 0.5 mg/l NAA in combination with 0.05 mg/l BAP produced the highest mean number of shoots per explants (10 ± 0.1). The shoot multiplication study is in progress and will be reported in a future paper.

Key words: Labisia pumila, shoot induction

 

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