Malays. Appl. Biol. (2016) 45(1): 81-87
EFFECTS OF DIETARY SUPPLEMENTATION OF LYSINE AND METHIONINE IN TEMPEH-BASED DIET ON GROWTH PERFORMANCE AND FEED UTILIZATION OF Epinephelus fuscoguttatus JUVENILES
WEI-KANG CHOR, LEONG-SENG LIM and ROSSITA SHAPAWI*
Borneo Marine Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88450, Kota Kinabalu, Sabah, Malaysia
Tel: +6088320121; Fax: +6088320261
*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
ABSTRACT
The potential of tempeh (TMP) with supplementation methionine (Met) and lysine (Lys) as a substitute of fishmeal (FM) was evaluated based on the growth performance and feed utilization for tiger grouper (Epinephelus fuscoguttatus) juveniles. Three diets were formulated to replace FM with TMP at 0% (D1, control diet), 40% without essential amino acids (EAAs) supplementation (D2) and 40% with EAAs supplementation (D3, 0.5% of Met and 0.5% of Lys) and fed to triplicate groups of fish (22.90±0.48g) twice a day for 8 weeks. Weight gain (WG), specific growth rate (SGR) and feed intake (FI) of D1 group (114.31%, 1.59%, and 45.51 g fish-1, respectively) were significantly higher than those fed with TMP-based diets (P<0.05). On the other hand, supplementation of EAAs in D3 was able to significantly improve the protein efficiency ratio (PER) and net protein utilization (NPU) (1.17% and 23.13%, respectively) compared to other diets (P<0.05). Supplementation of EAAs reduced whole-body lipid (P<0.05) but slightly increased whole body protein (P>0.05) of the fish. In the present study, results indicated that supplementation of EAAs was only able to improve feed utilization (PER and NPU) but not in growth performance (WG and SGR) of E. fuscoguttatus fed on TMP-based diets.
ABSTRAK
Potensi tempeh (TMP) yang ditambah dengan metionin (Met) dan lisin (Lys) sebagai pengganti tepung ikan (FM) dalam diet juvenil kerapu harimau (Epinephelus fuscoguttatus) telah diuji berdasarkan tumbesaran dan kecekapan permakanan. Tiga diet telah diformulasi untuk menggantikan FM dengan menggunakan TMP pada kadar 0% (D1, diet kawalan), 40% tanpa tambahan asid amino perlu (EAAs) (D2), dan 40% ditambah EAAs (D3, 0.5% of Met and 0.5% of Lys) dan diberi makan kepada kumpulan triplikat ikan (22.93±0.50g) dua kali sehari selama 8 minggu. Pertambahan berat (WG), kadar pertumbuhan spesifik (SGR) dan pengambilan makanan (FI) dalam kumpulan D1 adalah lebih tinggi secara signifikan (114.31%, 1.59%, dan 45.51 g ikan-1) daripada diet berasaskan TMP yang lain (P<0.05). Sebaliknya, tambahan EAAs dalam D3 telah meningkatkan kadar kecekapan protein (PER) dan penggunaan protein bersih (NPU) (masing-masing 1.17% dan 23.13%) secara signifikan berbanding diet-diet lain (P< 0.05). Penambahan EAAs telah mengurangkan kandungan lipid badan (P<0.05) tetapi serba sedikit meningkatkan kandungan protein badan (P>0.05). Keputusan kajian ini menunjukkan bahawa penambahan EAAs hanya mampu meningkatkan kecekapan makanan (PER dan NPU) tetapi tidak dalam peningkatan tumbesaran (WG dan SGR) E. fuscoguttatus yang diberi diet berasaskan TMP.
Key words: Epinephelus fuscoguttatus, Tempeh, methionine, lysine, growth performance, feed utilization
