Malays. Appl. Biol. (2017) 46(3): 33–40
OPTIMISATION OF ENZYMATIC PROTEIN HYDROLYSIS
OF MUD CRAB (Scylla sp.) TO OBTAIN MAXIMUM
ANGIOTENSIN-CONVERTING ENZYME INHIBITORY (ACEI)
ACTIVITY USING RESPONSE SURFACE METHODOLOGY
ZALIHA HARUN, AMIZA MAT AMIN*, NORIZAH MHD SARBON and
MOHAMAD KHAIRI MOHD ZAINOL
School of Food Science and Technology, Universiti Malaysia Terengganu (UMT),
21030 Kuala Nerus, Terengganu, Malaysia
*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 10 August 2017, Published online 4 October 2017
ABSTRACT
Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex® gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65ºC), pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum conditions were obtained at 65ºC, pH 5.6, 1% E/S ratio and 4 hours of hydrolysis time. The experimental ACEI activity (88.93±1.02%) was not significantly difference (p>0.05) with the predicted ACEI activity (91.10%). It was found that the IC50 of the mud crab hydrolysate prepared at optimum condition was 1.96±0.13 mg/mL. This study shows that RSM can be used to explain the relationship between enzymatic hydrolysis conditions of mud crab meat and its ACEI activity.
Key words: Mud crab, Enzymatic hydrolysis, protein hydrolysate, Response Surface Methodology (RSM), angiotensin-converting enzyme
