Malays. Appl. Biol. (2017) 46(3): 59–65
BIOFILM FORMATION OF Salmonella enterica AND
THE CENTRAL ROLE OF RPOS SIGMA FACTOR IN
STRESS RESISTANCE
WAN ZAWIAH WAN ABDULLAH1,2*, BERNARD M. MACKEY1 and
KIMON-ANDREAS G. KARATZAS1
1Department of Food and Nutrition Sciences, University of Reading,
P.O. Box 226, Whiteknights, Reading RG6 6AP, UK
2School of Food Science and Technology, Universiti Malaysia Terengganu,
21030, Kuala Nerus, Terengganu, Malaysia
*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 23 August 2017, Published online 4 October 2017
ABSTRACT
Non-typhoidal Salmonella is considered as the leading cause of foodborne illness and it has been associated with highprofile outbreaks in many groups of foods. This work examines the contribution of phenotypic properties related to survival (biofilm formation) and how these are linked with the genetic and functional variability of rpoS gene and RpoS status respectively in Salmonella enterica. The test strains were Salmonella serovars Anatum, Enteritidis (466), Enteritidis (496), Hadar, Heidelberg, Montevideo, Newport and Virchow and two Typhimurium strains previously characterised as either RpoSpositive or RpoS-negative. RpoS status was found to affect biofilm formation. The capability of Salmonella to resist stress and survive under unfavourable conditions can vary between strains. We confirmed that the two strains that were previously sensitive to various stresses harboured significant mutations in the rpoS gene. The rpoS sequencing not only confirmed a link between RpoS and biofilm formation, but it also revealed a link with differences in the utilisation of carbon sources. The RpoS-negative phenotype was linked with an increased growth under different carbon sources suggesting that a functional RpoS is a burden for growth which is in agreement with the SPANC hypothesis.
Key words: Salmonella enterica, biofilm, RpoS
