Malays. Appl. Biol. (2017) 46(4): 103–109
DIFFERENT SLAUGHTERING TECHNIQUES AND POSSIBLE
PHYSIOLOGICAL AND BIOMOLECULAR EFFECTS
RAHELA ZAMAN1, INTAN AZURA SHAHDAN2, SYED ZAHIR IDID2, NAFIU BIDEMI ABDURAZAQ1
and MOHAMMAD TARIQUR RAHMAN1,3*
1Faculty of Science, International Islamic University Malaysia (IIUM), Jalan Istana,
Bandar Indera Mahkota, 25200, Kuantan, Malaysia
2Faculty of Allied Health Sciences, IIUM, Jalan Istana, Bandar Indera Mahkota,
25200, Kuantan, Malaysia
3Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 10 August 2017, Published online 31 December 2017
ABSTRACT
The aim of the study was to identify possible physiological and biomolecular changes during slaughtering. For slaughtering, before the neck cutting, chickens are immobilized manually or immobilized using shackles. Neck cutting is generally performed using automated knife, often results in decapitation. Both of these immobilization and neck cutting conditions are expected to influence muscle contraction and blood loss. We have investigated the activity and transcription of acetylcholinesterase (AChE) which terminates cholinergic synaptic transmission by hydrolysing the neurotransmitter acetylcholine that is responsible for muscle contraction and relaxation. We have also analysed the residual haemoglobin content of the skeletal muscle as indicator of blood loss. Skeletal muscle sample was collected from the chickens that were slaughtered either by decapitation (C) or by severance of the jugular veins, carotid arteries, oesophagus and trachea only (P); whilst immediately after slaughtering, chickens were either released (R) or manually constrained (T). Differences in the conditions of slaughtering: CR, PR or PT did not affect blood loss as measured by the residual Hb content and the amount of Zn and Fe either in muscle or liver, deducing no significant difference (p>0.05) in blood loss due to different type of slaughtering. No significant differences (p>0.05) were observed in AChE activity in muscles taken from all slaughter groups. However, AChE transcripts were detected in muscles from chickens from PT and CR groups which might be due to the decapitation and/or constrained muscular activity after neck cutting. Hence the results of the current study indicate that, constraining during slaughtering and decapitation might induce stress to the animals.
Key words: Motor neuron, neuromuscular junction, acetylcholine, stress, haemoglobin, central nervous system
