Malays. Appl. Biol. (2018) 47(2): 9–13
FULL FACTORIAL DESIGN FOR PRODUCTION OF METHYL
3-(3,5-DI-TERT-BUTYL-4-HYDROXYPHENYL) PROPIONATE
USING OIL PALM FROND JUICE AS A SOLE SUBSTRATE
BY Ceratocystis fimbriata
NANG NOR AZIMAH LONG NADZRI1*, MIOR AHMAD KHUSHAIRI MOHD ZAHARI1,
SAIFUL NIZAM TAJUDDIN2 and CHE MOHD AIZAL CHE MOHD2
1Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang,
Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia
2Bio Aromatic Research Centre of Excellence, Universiti Malaysia Pahang, Lebuhraya Tun Razak,
26300 Kuantan, Pahang, Malaysia
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 16 March 2018, Published online 25 May 2018
ABSTRACT
Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration of glucose (g/L) on the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate from OPF juice by using Ceratocystis fimbriata (C. fimbriata). The design of experiment (DOE) method was used to screen the best parameters affecting the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The Gas Chromatography-Mass Spectroscopy (GC-MS) with Solid Phase Micro Extraction (SPME) was used to analyze and separate the peak of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. Based on the GC-MS analysis, results showed that the most favorable condition for methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate production was achieved at an initial pH medium (8), agitation speed (100 rpm), temperature (25°C) and 30 g/L of glucose in OPF juice. Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate were produced when the retention time was 32.80 min, the relative peak area was 0.24 % of chromatogram area. This result showed the great potential usage of OPF juice as a substrate to produce methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate by C. fimbriata.
Key words: Oil palm frond juice, Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate, Ceratocystis fimbriata.
