Malays. Appl. Biol. (2018) 47(3): 109–114
EFFECTS OF EXPLANT TYPES AND BASAL MEDIA ON CALLUS
INDUCTION OF Ficus deltoidea Jack var kunstleri (King) Corner
ZAKIAH MUSTAPHA1, TAJUL AFIF ABDULLAH2, DHIYA DALILA ZAWAWI2,
ABDUL JAMIL ZAKARIA2 and NOR HASIMA MAHMOD1,2*
1AGROPOLIS UniSZA, Universiti Sultan Zainal Abidin, Kampus Besut,
22200 Besut Terengganu. Malaysia
2Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin,
Kampus Besut, 22200 Besut Terengganu, Malaysia
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 25 May 2018, Published online 30 June 2018
ABSTRACT
Ficus deltoidea has been traditionally used as a folk medicine to treat various ailments such as diabetes and hypertension. Unsustainable harvesting and lack of replanting efforts have led to a severe reduction of this species. The present study was carried out to evaluate callus induction using F. deltoidea var. kunstleri (King) Corner by inoculating leaf, petiole, stamen and ovary explants in various kind of basal media augmented with 1 mg/L 2,4-D + 0.5 mg/L kinetin. It was found that callus formation varied significantly between explant types with the highest callus induction percentage shown by leaf segments. The leaf explant showed callus formation in all basal media with Murashige and Skoog (MS) showing the highest callus induction percentage (63.88% ± 5.55) followed by modified Murashige and Skoog (Cb) (47.22% ± 5.55), Bourgin and Nitsch (BN) (47.22% ± 2.77) and Lloyd and Mc Cown (LM) (38.89% ± 5.55). The findings from the current study provide the basis of callus culture protocol of Ficus deltoidea Jack var kunstleri (King) Corner with leaf and MS being the most suitable explant and basal medium respectively.
Key words: Ficus deltoidea, callus induction, basal media, explant, growth regulators
