Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2009) 38(1): 1–9


School of Biosciences and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia. E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


In repeated batch culture, the cultures were able to maintain their productivity higher than that obtained in batch culture where the highest lipid content and concentration were achieved at 34.36% (g lipid/g biomass) and 3.02 g/L, respectively, compared to that obtained in batch culture at 27.39% (g lipid/g biomass) and 1.87 g/L, respectively. The highest gamma-linolenic acid (GLA) content and concentration were 10.54% of total lipid and 0.28 g/L, respectively, compared to that obtained in batch culture at 9.81% and 0.16 g/L, respectively. The highest maximum biomass concentration was 14.97 g/L. This was achieved using nitrogen limited medium consisting of glucose as the carbon source and ammonium tartrate as the nitrogen source. Lipid concentration increased 14.11% after the second repeat and continued to 45.40% in the fourth repeat after 300 hr of fermentation. Therefore, cumulative productivities of lipid in each cycle of a repeated batch culture increased with increasing number of cycles. The cumulative productivity of lipid and GLA were 0.0260 g/L/hr and 0.0024 g/L/hr, respectively, which were 3.02 fold and 3.43 fold higher than that achieved from batch culture.


Kultur kelompok ulangan mampu menyelenggara produktiviti yang lebih tinggi berbanding kultur kelompok. Kandungan dan kepekatan lipid tertinggi dicapai sebanyak 34.36% (g lipid/g biojisim) dan 3.02 g/L masing-masing berbanding dengan yang diperoleh dalam kultur kelompok hanya sebanyak 27.39% (g lipid/g biojisim) dan 1.87 g/L masing-masing. Kandungan dan kepekatan GLA tertinggi adalah sebanyak 10.54% daripada lipid total dan 0.28 g/L masing-masing berbanding dengan yang diperoleh dalam kultur kelompok sebanyak 9.81% daripada lipid total dan 0.16 g/L masing-masing. Kepekatan biojisim maksimum yang tertinggi adalah sebanyak 14.97 g/L. Kesemua data dicapai dengan menggunakan medium terhad nitrogen yang mengandungi glukosa sebagai sumber karbon dan ammonium tartarat sebagai sumber nitrogen. Kepekatan lipid meningkat sebanyak 14.11% diakhir ulangan kedua dan terus meningkat sehingga mencapai 45.40% diakhir ulangan keempat selepas 300 j fermentasi. Oleh itu, produktiviti kumulatif lipid dalam setiap ulangan kultur kelompok ulangan meningkat mengikut jumlah ulangan. Produktiviti kumulatif lipid dan GLA dalam kultur kelompok ulangan adalah 0.0260 g/L/j dan 0.0024 g/L/j masing-masing dan adalah lebih tinggi daripada pencapaian kultur kelompok sebanyak 3.02 dan 3.43 ganda.


Abdul Hamid, A., W. Yusoff, W.M., M. Illias, R. & Nadarajah, K. 2001. Pengasingan strain baru kulat dari tanah di Malaysia untuk penghasilan asid- gamma linolenik (GLA). Jurnal Teknologi34:1-8.

Chaney, A.L. & Marbach, E.P. 1962. Modified reagents for the determination of ammonium and urea. Clinical Chemistry8:130-132.

De, B.K., Chaudhury, S. & Bhattacharyya, D.K. 1999. Effect of nitrogen sources on gamma-linolenic acid accumulation in Spirulina platensiJournal of the American Oil Chemists’ society76(1):153-156.

Folch, J., Lees, M. & Sloane-Stanley, G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry226:497-509.

Fukuda, H. & Morikawa, H. 1987. Enhancement of gamma-linolenic acid production by Mucor ambiguouswith nonionic surfactants. Applied Microbiology Biotechnolology, 27:15-20.

Hansson, L. & Dostalek, M. 1988. Effect of culture conditions on mycelial growth and production of gamma-linolenic acid by the fungus Mortierella ramannianaApplied Microbiology Biotechnology28:240-246.

Hansson, L., Dostalek, M. & Sorenby, B. 1989. Production of gamma-linolenic acid by the fungus Mucor rouxiiin fed-batch and continuous culture. Applied Microbiology Biotechnology31:223-227.

Hirano, M., Mori, H. & Matsunaga, T. 1990. Gamma-linolenic acid production by micro-algae.Applied Microbiology Biotechnology24­-25:183-191.

Huang, W.C., Chen, S.J. & Chen, T.L. 2008. Production of hyaluronic acid by repeated batch fermentation. Biochemical Engineering Journal40:460-464.

Jang, M.Y., Ryu, W.R. & Cho, M.H. 2002. Laccase production from repeated batch cultures using free mycelia of Trametessp. Enzyme and Microbial Technology30:741-746.

Kendrick, A. & Ratledge, C. 1992. Lipids of selected molds grown for production of n-3 and n-6 polyunsaturated fatty acids. Lipids27:15-20.

Kleiman, R. 1988. Genetic diversity of lipids in plant germplasm. Ibid., 73-77.

Lin, Y.C., Wu, J.Y. & Chen, T.L. 2001. Production of Acinetobacter radioresistenslipase with repeated batch culture in presence of nonwoven fabric. Biotechnology Bioengineering76:214­-218.
Naghmouchi, K., Fliss, I., Drider, D. & Lacroix, C. 2008. Pediocin PA-1 production during repeated-cycle batch culture of immobilized Pediococcus acidilacticiUL5 cells. Journal of Bioscience and Bioengineering105(5):513-­517.

Naritomi, T., Kouda, T., Yano, H. & Yoshinaga, F. 2002. Influence of broth exchange ratio on bacterial cellulose production by repeated batch culture. Process Biochemistry38:41-47.

Sakai, S., Nakashimada, Y. & Inokuma, K. 2005. Acetate and ethanol production from H2 and CO2 by Moorellasp. using a repeated batch culture. Journal of Bioscience and Bioengineering99(3):252-258.

Shakeri, M., Sugano, Y. & Shoda, M. 2007. Production of Dye-Decolorizing Peroxidase (rDyP) from complex substrates by repeated batch and fed-batch cultures of recombinant Aspergillus oryzae. 103(2):129-134.

Srinivasan, K., Murakami, M., Nakashimada, Y. & Nishio, N. 2001. Efficient production of cellulolytic and xylanolytic enzymes by the rumen anaerobic fungus, Neocallimastix frontalis, in a repeated batch culture. Journal of Bioscience and Bioengineering91(2):153-158.

Stanbury, P.F., Whitaker, A. & Hall, S.J. 1995. Principles of fermentation technology. 2nd Ed. London: Elsevier Science Ltd. 13pp.

Wan, H.M., Chen, C.C., Giridhar, R. & Chang, T.S. 2005. Repeated batch production of kojic acid in a cell-retention fermenter using Aspergillus oryzaeM3B9. Journal Ind Microbiology Biotechnology32:227-233.

Yang, X., Wang, B., Cui, F. & Tan, T. 2005. Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizusProcess Biochemistry40:2095-2103.

Yin, P., Yahiro, K., Ishigaki, T., Park, Y. & Okabe,M. 1998. L(+)-lactic acid production by repeated batch culture of Rhizopus oryzaein air-lift bioreactor. Journal of Fermentation and Bioengineering85(1):96-100.



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