Malays. Appl. Biol. (2009) 38(1): 1–9
ENHANCED PRODUCTIVITY OF LIPID AND GAMMA LINOLENIC ACID BY Cunninghamella bainieri 2A1 IN REPEATED BATCH CULTURES
MUSLEEHAWATEE WORNGOR, AIDIL ABDUL HAMID and WAN MOHTAR WAN YUSOFF*
School of Biosciences and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia. E-mail:
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ABSTRACT
In repeated batch culture, the cultures were able to maintain their productivity higher than that obtained in batch culture where the highest lipid content and concentration were achieved at 34.36% (g lipid/g biomass) and 3.02 g/L, respectively, compared to that obtained in batch culture at 27.39% (g lipid/g biomass) and 1.87 g/L, respectively. The highest gamma-linolenic acid (GLA) content and concentration were 10.54% of total lipid and 0.28 g/L, respectively, compared to that obtained in batch culture at 9.81% and 0.16 g/L, respectively. The highest maximum biomass concentration was 14.97 g/L. This was achieved using nitrogen limited medium consisting of glucose as the carbon source and ammonium tartrate as the nitrogen source. Lipid concentration increased 14.11% after the second repeat and continued to 45.40% in the fourth repeat after 300 hr of fermentation. Therefore, cumulative productivities of lipid in each cycle of a repeated batch culture increased with increasing number of cycles. The cumulative productivity of lipid and GLA were 0.0260 g/L/hr and 0.0024 g/L/hr, respectively, which were 3.02 fold and 3.43 fold higher than that achieved from batch culture.
ABSTRAK
Kultur kelompok ulangan mampu menyelenggara produktiviti yang lebih tinggi berbanding kultur kelompok. Kandungan dan kepekatan lipid tertinggi dicapai sebanyak 34.36% (g lipid/g biojisim) dan 3.02 g/L masing-masing berbanding dengan yang diperoleh dalam kultur kelompok hanya sebanyak 27.39% (g lipid/g biojisim) dan 1.87 g/L masing-masing. Kandungan dan kepekatan GLA tertinggi adalah sebanyak 10.54% daripada lipid total dan 0.28 g/L masing-masing berbanding dengan yang diperoleh dalam kultur kelompok sebanyak 9.81% daripada lipid total dan 0.16 g/L masing-masing. Kepekatan biojisim maksimum yang tertinggi adalah sebanyak 14.97 g/L. Kesemua data dicapai dengan menggunakan medium terhad nitrogen yang mengandungi glukosa sebagai sumber karbon dan ammonium tartarat sebagai sumber nitrogen. Kepekatan lipid meningkat sebanyak 14.11% diakhir ulangan kedua dan terus meningkat sehingga mencapai 45.40% diakhir ulangan keempat selepas 300 j fermentasi. Oleh itu, produktiviti kumulatif lipid dalam setiap ulangan kultur kelompok ulangan meningkat mengikut jumlah ulangan. Produktiviti kumulatif lipid dan GLA dalam kultur kelompok ulangan adalah 0.0260 g/L/j dan 0.0024 g/L/j masing-masing dan adalah lebih tinggi daripada pencapaian kultur kelompok sebanyak 3.02 dan 3.43 ganda.
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