Malays. Appl. Biol. (2019) 48(3): 105–111
ANTI-VIRAL ACTIVITY OF Phyllanthus niruri AGAINST
HEPATITIS C VIRUS
TUTIK SRI WAHYUNI1,2*, DZUL AZMI1, ADITA AYU PERMANASARI2, MYRNA ADIANTI2,4,
LYDIA TUMEWU2, TRI WIDIANDANI3, CHIE AOKI UTSUBO5, ATY WIDYAWARUYANTI1,2,
ACHMAD FUAD1,2 and HAK HOTTA6
1Department of Pharmacognocy and Phytochemistry, Faculty of Pharmacy,
Airlangga University, Surabaya 60115, Indonesia
2Institute of Tropical Disease, Airlangga University, Surabaya, 60115, Indonesia
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy,
Airlangga University, Surabaya 60115, Indonesia
4Tradisional Medicine Study Program, Faculty of Vocational Studies,
Airlangga University, Surabaya 60115, Indonesia
5Department of International Health, Kobe University Graduate School of Health Sciences,
7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, Japan
6Faculty of Clinical Nutrition and Dietetics, Konan Women’s University,
6-2-23 Morikita-machi, Higashinada-ku, Kobe 658-0001, Japan
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 21 May 2019, Published online 30 June 2019
ABSTRACT
Hepatitis C virus (HCV) infection is a global problem that causes liver disease and hepatocellular carcinoma. Although the current standard treatment provided a significant improvement on response rate with sustain virology response more than 90%, however, the high cost was remaining limited access to this therapy, resistance emergence and serious side effects which provide the necessities to find the new anti-HCV agents. The current study, we evaluated the ethanol extract of Phyllanthus niruri for its anti-HCV activities. Anti-HCV activity was determined by in vitro culture cells of Huh 7it. Anti-HCV activity of P. niruri extract revealed strong inhibition against HCV with IC50 values of 4.14 ?g/mL and yield stronger activity in the entry step of the HCV life cycle. Moreover, the P. niruri extract enhanced anti-HCV activity of simeprevir (NS3 protease inhibitor) with increase the activity up to 4-fold compared to a single treatment of simeprevir. Docking analysis was performed to predict the interaction phyllanthin and hypophyllantin, known compounds of P. niruri against HCV receptor. Both of phyllantin and hypophyllantin were mediated a strong interaction with 4GAG, a protein that involved in entry step of HCV. These results suggested that the ethanol extract of P. niruri may be good candidates for the development of anti-HCV drugs.
Key words: Hepatitis C virus, antiviral activity, Phyllanthus niruri, entry step
