Malays. Appl. Biol. (2020) 49(1): 61–68
GENETIC DIVERSITY OF GREEN CHIRETA
(Andrographis paniculata (Burm.f.) Wall. Ex Nees.)
FROM INDONESIA BASED ON ISSR AND RAPD MARKERS
MUHAMMAD FAUZI ARIF1, DYAH SUBOSITI2, ANISKA NOVITA SARI2,
GANIES RIZA ARISTYA3, INDRA LESMANA3 and RINA SRI KASIAMDARI4*
1Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada,
Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
2Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional,
Lawu Street No. 11, Tawangmangu, Karanganyar, Central Java 57792, Indonesia
3Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada,
Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
4Laboratory of Plant Systematics, Faculty of Biology, Universitas Gadjah Mada,
Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 15 February 2020, Published online 30 June 2020
ABSTRACT
Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were used, DNA was amplified using six ISSR and six RAPD primers, generating 61 loci and 43 loci respectively. Genotype data scoring resulted in the percentage of polymorphism, Polymorphic Information Content, Shannon’s Information Index and unbiased expected heterozygosity. The genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei’s unbiased genetic distance. The data from this study revealed that the variation and diversity from accessions within the same island were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance (AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The results showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity provided information to help the further development and cultivation of A. paniculata in Indonesia.
Key words: Andrographis paniculata, genetic diversity, ISSR, medicinal plant, RAPD
