.Malays. Appl. Biol. (2009) 38(1): 55–60
CHARACTERIZATION OF CHITOSAN FROM Absidia butleri dr.
KALAIVANI NADARAJAH1 and ABDUL JALIL ABDUL KADER2
1School of Biosciences and Biotechnology, Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, 43600 UKM. Bangi, Selangor Darul Ehsan.
2 Faculty of Science and Technology, Islamic Science University of Malaysia,
71800 Bandar Baru Nilai, Negeri Sembilan, Malaysia
Coressponding Author:
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ABSTRACT
The Absidia butleri drwas grown in two types of medium with different nitrogen sources. The highest yield of chitosan (Y/200mL 0.092g) was obtained in Yeast-Peptone Glucose (YPG) supplemented with peptone as its organic nitrogen source. YPG medium produced chitosan with higher molecular weight (22.7 x 104 Da) and lower degree of deacetylation (88%) compared to the chitosan obtained from nitrogen limiting medium (NLM)(5.1 x 104 Da and 90%). When compared to crustacean chitosan (Chitochem) (2.346 x 106 Da and 84%), fungal chitosan had a lower molecular weight (22.7 – 5.1 x 104 Da) and a higher degree of deacetylation (84 – 90%). Scanning electron microscopy (SEM) conducted on fungal chitosan obtained from YPG and NLM showed that the chitosan obtained from YPG medium had larger particle size compared to particle size from NLM medium. The microparticle size increased proportionally with the increase of the molecular weight.
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