Malays. Appl. Biol. (2020) 49(1): 121–130
PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND
ANTIBACTERIAL ACTIVITIES OF EXTRACTS AND
FRACTIONS OF Dillenia suffruticosa LEAVES
FARAZIMAH YAKOP1,3, MALAI HANITI SHEIKH ABDUL HAMID1,3*, NORHAYATI AHMAD2,3,
MOHAMED ABDUL MAJID2,3,4, MANOHARAN KARUPPIAH PILLAI3,5 and HUSSEIN TAHA2,3
1Chemical Sciences, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link,
Gadong BE1410, Brunei Darussalam
2Environmental and Life Sciences, Faculty of Science, Universiti Brunei Darussalam,
Jalan Tungku Link, Gadong BE1410, Brunei Darussalam
3Herbal Drug Discovery Laboratory, Faculty of Science, Universiti Brunei Darussalam,
Jalan Tungku Link, Gadong BE1410, Brunei Darussalam
4Rimba Ilmu, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
5Faculty of Science and Technology, Department of Chemistry and Chemical Technology,
National University of Lesotho, P.O Roma 180, Kingdom of Lesotho, South Africa
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 24 May 2020, Published online 30 June 2020
ABSTRACT
Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant and antibacterial activities of aqueous extract, methanol extract and its fractions obtained from the leaves of D. suffruticosa. The dried leaves were extracted using methanol before successive solvent partitioning was carried out on the extract using hexane, chloroform, ethyl acetate and diethyl ether. In addition to this, aqueous decoction was conducted. The antioxidant activities were determined using Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging methods. Phytochemical screening had shown that most of the extracts and fractions contained alkaloids, steroids, phenolics, flavonoids and saponins. The diethyl ether and ethyl acetate fractions showed higher TPC and TFC values. The diethyl ether and ethyl acetate fractions also showed higher antioxidant activities determined via DPPH assay. Antibacterial activities determined using disc diffusion assay showed the methanol extract and its fractions had antibacterial activity against Staphylococcus aureus, with the diethyl ether fraction having comparable activity with the standard antibiotic streptomycin. However, inhibition against Bacillus subtilis was only observed in hexane, chloroform and diethyl ether fractions. No inhibition was observed against Escherichia coli and Pseudomonas aeruginosa. This study identified the diethyl ethyl and ethyl acetate fractions of D. suffruticosa leaves as potential sources of bioactive compounds. Further investigations on the bioassay-guided isolation of bioactive compounds of this species may lead to the discovery of new pharmaceutical or effective antimicrobial agents.
Key words: Dillenia suffruticosa, Dilleniaceae, antioxidant activity, antibacterial activity, flavonoids, phenolics, phytochemical screening
