Malays. Appl. Biol. (2020) 49(1): 153–158
CHARACTERISATION OF AmCCR1 AND pseudoCOMTAm
PROMOTERS IN Acacia mangium
AZMAH ABDUL LATIF1, SHEH MAY TAM2 and WICKNESWARI RATNAM1*
1School of Environmental and Natural Resource Sciences, Faculty of Science and Technology,
Universiti Kebangsaan Malaysia, 43650 Bangi Selangor, Malaysia
2School of Biosciences, Taylor’s University
*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Accepted 13 June 2020, Published online 30 June 2020
ABSTRACT
Acacia mangium is one of the most important species in forest plantation industry in Southeast Asia. Lignin is a complex polymer found in plant which has to be removed using harsh and toxic chemicals for the production of high quality paper. Understanding the complex pathway that underlies the regulation of lignin biosynthetic genes requires in depth knowledge of the genes involved and it’s regulatory elements. Using Thermal Asymmetric Interlaced PCR, a 770bp promoter of pseudoCOMTAm and 619bp of AmCCR1 promoter were isolated. Bioinformatics analysis revealed the presence of cis acting elements commonly found in lignin biosynthetic genes such as TATA box, CAAT box, W box, AC-I and AC-11 elements on both promoters.
Key words: Acacia mangium, AmCCR1 promoter, pseudoCOMTAm promoter, sequence analysis
